Cationic methacrylate polymers as topical antimicrobial agents against Staphylococcus aureus nasal colonization.
Cationic methacrylate polymers as topical antimicrobial agents against Staphylococcus aureus nasal colonization.
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DOI：
10.1021/bm500557d
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发表时间：
2014-08-11
影响因子：
6.2
通讯作者：
Kuroda, Kenichi
中科院分区：
文献类型：
--
作者：
Thoma, Laura M.;Boles, Blaise R.;Kuroda, Kenichi
研究了甲基丙烯酸乙酯伯铵均聚物 (AEMP) 的体外和体内抗菌活性。通过可逆加成断裂链转移（RAFT）聚合制备了不同聚合度（DP = 7.7-12）的AEMP。 AEMP对革兰氏阳性菌（包括耐甲氧西林金黄色葡萄球菌（MRSA））比革兰氏阴性菌表现出更高的抑制作用。 AEMP 还显示出有效的抗 S.在胎牛血清存在下金黄色葡萄球菌的活性，而抗生素莫匹罗星的活性在相同条件下降低。 AEMP 显示出很少或没有溶血活性。 AEMP 对哺乳动物细胞 HEp-2 和 COS-7 的细胞毒性具有浓度依赖性，并且在较高的聚合物浓度下细胞活力显着降低。与对照组相比，AEMP 显着减少了棉鼠鼻腔环境中活的金黄色葡萄球菌细胞的数量。这项研究表明 AEMP 有潜力用于治疗局部金黄色葡萄球菌感染。
The in vitro and in vivo antimicrobial activity of primary ammonium ethyl methacrylate homopolymers (AEMPs) was investigated. AEMPs with different degrees of polymerization (DP = 7.7–12) were prepared by reversible addition–fragmentation chain-transfer (RAFT) polymerization. The AEMPs showed higher inhibitory effects against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), than Gram-negative bacteria. The AEMPs also showed potent anti-S. aureus activity in the presence of fetal bovine serum, whereas the activity of the antibiotic mupirocin was reduced under the same conditions. The AEMPs showed very little or no hemolytic activity. The cytotoxicity of AEMPs against mammalian cells HEp-2 and COS-7 was concentration-dependent, and the cell viability significantly decreased at higher polymer concentrations. The AEMPs significantly reduced the number of viable S. aureus cells in the nasal environment of cotton rats when compared to that of the control. This study demonstrates that AEMPs have potential for use in treating topical S. aureus infections.
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影响因子：
6.2
作者：
King, Allison;Chakrabarty, Souvik;Zhang, Wei;Zeng, Xiaomei;Ohman, Dennis E.;Wood, Lynn F.;Abraham, Sheena;Rao, Raj;Wynne, Kenneth J.
通讯作者：
Wynne, Kenneth J.
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影响因子：
5.5
作者：
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通讯作者：
Yang, Lihua
影响因子：
3.7
作者：
Al-Ahmad A;Laird D;Zou P;Tomakidi P;Steinberg T;Lienkamp K
通讯作者：
Lienkamp K
影响因子：
4.6
作者：
Gibney, Katherine A.;Sovadinova, Iva;Lopez, Analette I.;Urban, Michael;Ridgway, Zachary;Caputo, Gregory A.;Kuroda, Kenichi
通讯作者：
Kuroda, Kenichi
影响因子：
3.4
作者：
Epand, Raquel F.;Sarig, Hadar;Epand, Richard M.
通讯作者：
Epand, Richard M.