PHYLOGENY OF FOUR DINOPHYSIACEAN GENERA (DINOPHYCEAE, DINOPHYSIALES) BASED ON rDNA SEQUENCES FROM SINGLE CELLS AND ENVIRONMENTAL SAMPLES
PHYLOGENY OF FOUR DINOPHYSIACEAN GENERA (DINOPHYCEAE, DINOPHYSIALES) BASED ON rDNA SEQUENCES FROM SINGLE CELLS AND ENVIRONMENTAL SAMPLES
复制标题
DOI：
10.1111/j.1529-8817.2009.00738.x
复制
发表时间：
2009-10-01
影响因子：
2.9
通讯作者：
Delwiche, Charles F.
中科院分区：
文献类型：
--
作者：
Handy, Sara M.;Bachvaroff, Tsvetan R.;Delwiche, Charles F.
甲藻是一个高度多样性和环境重要的原生生物群体，系统发育关系的分辨率相对较低，特别是在异养物种之间。我们研究了几个甲藻纲甲藻的繁殖利用从四个大西洋网站收集的样品。作为一项规则，3.5 kb的序列，包括核核糖体基因SSU，5.8S，LSU，加上他们的内部转录间隔区（ITS）1和2区确定的26个个体，包括两个属的代表，分子数据以前是不可用的，鸟尾丝虫F。Stein和Histoneis F.斯坦此外，一个克隆文库的目标甲藻ITS 2和LSU序列构建从散装环境DNA从三个网站。从数据中推断出三个系统发育树，一个使用本研究的数据，用于确定属或种的细胞（3.5kb，28个分类群）;另一个包含来自GenBank的甲藻SSU提交物和12个新的甲藻纲序列（1.9kb，56个分类单元）;第三棵树结合了来自已鉴定分类群、甲藻纲GenBank提交资料和本研究克隆文库的数据（2.1 kb，136个分类群）。所有的树都是一致的，并表明一个明显的划分属Phalacroma F。Stein和Dinophysis Escherichia b.含组囊藻属和鸟尾藻属的蓝藻也是单系的。这是迄今为止进行的最大的甲藻类分类群的分子系统发育，并与Phalacroma属可能不是甲藻属的同义词的观点一致。
Dinoflagellates are a highly diverse and environmentally important group of protists with relatively poor resolution of phylogenetic relationships, particularly among heterotrophic species. We examined the phylogeny of several dinophysiacean dinoflagellates using samples collected from four Atlantic sites. As a rule, 3.5 kb of sequence including the nuclear ribosomal genes SSU, 5.8S, LSU, plus their internal transcribed spacer (ITS) 1 and 2 regions were determined for 26 individuals, including representatives of two genera for which molecular data were previously unavailable, Ornithocercus F. Stein and Histioneis F. Stein. In addition, a clone library targeting the dinophysiacean ITS2 and LSU sequences was constructed from bulk environmental DNA from three sites. Three phylogenetic trees were inferred from the data, one using data from this study for cells identified to genus or species (3.5 kb, 28 taxa); another containing dinoflagellate SSU submissions from GenBank and the 12 new dinophysiacean sequences (1.9 kb, 56 taxa) from this study; and the third tree combing data from identified taxa, dinophysiacean GenBank submissions, and the clone libraries from this study (2.1 kb, 136 taxa). All trees were congruent and indicated a distinct division between the genera Phalacroma F. Stein and Dinophysis Ehrenb. The cyanobionts containing genera Histioneis and Ornithocercus were also monophyletic. This was the largest molecular phylogeny of dinophysoid taxa performed to date and was consistent with the view that the genus Phalacroma may not be synonymous with Dinophysis.