Proposed standardization of Neisseria meningitidis PorA variable-region typing nomenclature
Proposed standardization of Neisseria meningitidis PorA variable-region typing nomenclature
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DOI：
10.1128/cdli.5.6.845-855.1998
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发表时间：
1998-11-01
期刊：
影响因子：
--
通讯作者：
Mayer, LW
中科院分区：
文献类型：
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作者：
Sacchi, CT;Lemos, APS;Mayer, LW
脑膜炎奈瑟菌分离物通常通过血清分型进行分类，其特征是PorA外膜蛋白可变区（VR）表位与单克隆抗体（mab）的反应性。一种较新的方法（PorA VR分型）使用来自DNA序列分析的预测氨基酸序列。由此产生的分类方案没有标准化，两种方法提供的数据相互冲突，有时甚至不可调和。在本文中，我们提出了一种标准化的PorA VR分型命名法，该命名法将传统的PorA血清分型的血清学信息与预测的VR序列的分子数据结合起来。我们进行了全面的文献和数据库检索，生成了一系列菌株和DNA序列，这些菌株和DNA序列反映了迄今为止存在的PorA的多样性。我们将这些信息安排在一个综合的逻辑模型中，包括血清亚型和PorA VR类型分配。我们的数据表明，目前的血清亚型定义单抗至少低估了50%的PorA VR变异性。我们对VR分型的建议是有意义的，因为氨基酸序列和血清学信息，当血清亚型定义单克隆抗体可用时，可以同时从PorA VR名称推断出来。该方案为今后脑膜炎奈索菌的分类和应用流行病学研究提供了一种系统的方法，可用于筛选候选疫苗和分析疫苗覆盖率和失败情况。
Neisseria meningitidis isolates are conventionally classified by serosubtyping, which characterizes the reactivities of the PorA outer membrane protein variable-region (VR) epitopes with monoclonal antibodies (MAbs). A newer method (PorA VR typing) uses predicted amino acid sequences derived from DNA sequence analysis. The resulting classification schemes are not standardized, offering conflicting and sometimes irreconcilable data from the two methods. In this paper, we propose a standardization of the PorA VR typing nomenclature that incorporates serologic information from traditional PorA serosubtyping with molecular data from predicted VR sequences. We performed a comprehensive literature and database search, generating a collection of strains and DNA sequences that reflects the diversity within PorA that exists to date. We have arranged this information in a comprehensive logical model that includes both serosubtype and PorA VR type assignments. Our data demonstrate that the current panel of serosubtype-defining MAbs underestimates PorA VR variability by at least 50%. Our proposal for VR typing is informative because amino acid sequence and serologic information, when serosubtype-defining MAbs are available, can be deduced simultaneously from the PorA VR designation. This scheme will be useful in future classification and applied epidemiologic studies of N. meningitidis, being a systematic way of selecting PorA vaccine candidates and analyzing vaccine coverage and failure.