Co-expression of surfactant protein A and chicken lung lectin in chicken respiratory system
Co-expression of surfactant protein A and chicken lung lectin in chicken respiratory system
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表面活性蛋白A与鸡肺凝集素在鸡呼吸系统中的共表达
DOI：
10.1016/j.molimm.2020.03.018
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发表时间：
2020
影响因子：
3.6
通讯作者：
Qi Kezong
中科院分区：
文献类型：
--
作者：
Liu Hongmei;Huang Xueting;Xiong Haifeng;Liu Miaomiao;Hu Dongmei;Wei Changqing;Wang Guijun;Qi Kezong
鸡表面活性蛋白A（cSP-A）和鸡肺凝集素（cLL）是在肺宿主防御反应中起重要作用的C型凝集素。本研究旨在探讨cSP-A和cLL在鸡呼吸系统中的定位。采用实时定量逆转录聚合酶链反应（qRT-PCR）和荧光多重免疫组织化学染色（荧光mIHC染色）对30日龄SPF鸡的6种组织中cSP-A和cLL的表达进行定量。结果表明，cSP-A和cLL mRNA在肺组织中的表达高于其他组织。如使用qRT-PCR分析的，与cLL表达水平相比，所有组织中的cSP-A mRNA表达水平更高。cSP-A和cLL的荧光mIHC共表达主要见于肺支气管旁上皮、喉、气管、空洞、支气管和气囊黏膜上皮，其中cSP-A的阳性染色较cLL强。cLL在两个粘膜表面上表达，一些个体肺上皮细胞和软骨细胞，而cSP-A主要限于呼吸道的粘膜表面。这些组织学结果可能有助于了解这种肺凝集素在未来研究中的生物学意义。
Chicken surfactant protein A (cSP-A) and chicken lung lectin (cLL) are C-type lectins that play important roles in pulmonary host defense responses. Herein, we explored the localization of cSP-A and cLL in the chicken respiratory system. Six tissues from 30-days-old SPF chickens were used to quantify the expression of cSP-A and cLL using the quantitative real-time reverse transcriptional polymerase chain reaction (qRT-PCR) and fluorescence multiplex immunohistochemistry staining (fluorescence mIHC staining). Results showed that cSP-A and cLL mRNA were highly expressed in lungs compared to other tissues. cSP-A mRNA expression levels in all tissues were higher compared with cLL expression levels as analyzed using qRT-PCR. Fluorescence mIHC co-expression of cSP-A and cLL were mainly detected in lung parabronchial epithelia, and mucosal epithelia of larynx, trachea, syrinx, bronchus and air sac, with cSP-A showing a stronger positive staining compared with cLL. cLL is expressed on both mucosal surfaces, some individual lung epithelial cells and cartilage cells, while cSP-A is mainly restricted to mucosal surfaces of the respiratory tract. These histological findings may be useful for understanding the biological significance of this pulmonary lectins in future studies.