CD62/P-SELECTIN RECOGNITION OF MYELOID AND TUMOR-CELL SULFATIDES
CD62/P-SELECTIN RECOGNITION OF MYELOID AND TUMOR-CELL SULFATIDES
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DOI：
10.1016/0092-8674(91)90570-o
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发表时间：
1991-10-04
期刊：
影响因子：
64.5
通讯作者：
SEED, B
中科院分区：
文献类型：
--
作者：
ARUFFO, A;KOLANUS, W;SEED, B
CD 62，也称为PADGEM蛋白、GMP-140或P-选择素，是内皮细胞和血小板的颗粒膜蛋白，在暴露于凝血酶、组胺、补体成分或过氧化物等介质后被动员到质膜。 迄今为止的数据表明，CD 62的一种配体包括CD 15（刘易斯x决定簇）和唾液酸。 我们在这里表明，硫苷脂，异质性3-硫酸半乳糖神经酰胺，是一个显然无关的配体的CD 62。 硫苷脂在粒细胞的质膜上表达并由粒细胞分泌，并且构成某些肿瘤细胞质膜上CD 62的主要配体。 CD 62与吸附在塑料上的硫苷脂结合，就像它与骨髓或肿瘤细胞结合一样。 我们发现，粒细胞分泌硫苷脂的速度预测，使他们能够迅速从CD 62释放，一旦他们已经离开血流。
CD62, also called PADGEM protein, GMP-140, or P-selectin, is a granule membrane protein of endothelial cells and platelets that is mobilized to the plasma membrane following exposure to mediators such as thrombin, histamine, complement components, or peroxides. Data presented to date suggest that one ligand of CD62 includes CD15 (Lewis x determinant) and sialic acid. We show here that sulfatides, heterogeneous 3-sulfated galactosyl ceramides, are an apparently unrelated ligand of CD62. Sulfatides are expressed on the plasma membrane of, and are excreted by, granulocytes, and constitute the principal ligand for CD62 on the plasma membrane of some tumor cells. CD62 binds to sulfatides adsorbed to plastic as avidly as it binds to myeloid or tumor cells. We find that granulocytes excrete sulfatides at a rate predicted to allow them to be rapidly released from CD62 once they have exited the bloodstream.