Frequent isolation of methicillin resistant Staphylococcus aureus (MRSA) ST398 among healthy pigs in Portugal.
Frequent isolation of methicillin resistant Staphylococcus aureus (MRSA) ST398 among healthy pigs in Portugal.
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DOI：
10.1371/journal.pone.0175340
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Aires-de-Sousa M
中科院分区：
文献类型：
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作者：
Conceição T;de Lencastre H;Aires-de-Sousa M
尽管与牲畜相关的 ST398 耐甲氧西林金黄色葡萄球菌 (MRSA) 在不同地理区域已被广泛报道，但葡萄牙健康猪中 MRSA 携带研究非常有限。总共对来自两个葡萄牙农场的 101 份猪鼻样本进行了 MRSA 筛查。此外，五名养猪工人（包括一名兽医和一名工程师）和四名家庭成员也接受了鼻腔筛查。通过 spa 分型、SCCmec 分型和 MLST 对分离株进行了表征。所有分离株均进行了抗菌敏感性、mecA 和 mecC 基因的存在以及毒力决定因素的测试。猪中 MRSA 患病率为 99% (100/101)，养猪工人中为 80% (4/5)，家庭成员中为 25% (1/4)。所有分离株均属于 ST398，分布在两种 spa 类型中：t011 (57%) 和 t108 (42%)。 SCCmec V 型存在于大多数分离株中（n = 95；82%），而 21 个分离株仅扩增 mecA 基因，被归类为不可分型。大多数分离株对四环素（100%）、克林霉素（97%）、红霉素（96%）、氯霉素（84%）和庆大霉素（69%）耐药。值得注意的是，12% 对奎努普丁-达福普丁表现出耐药性（MIC 3-8 μg/mL）。 β-溶血素 (81%) 和 γ-溶血素 (74%) 是检测到的独特毒力决定因素。没有一个分离株带有 PVL 或 mecC 基因。这项研究表明 ST398-MRSA 在两个独立的养猪场中大量出现，凸显了该病毒在葡萄牙健康猪中的存在。
Although livestock-associated ST398 methicillin-resistant Staphylococcus aureus (MRSA) has been widely reported in different geographic regions, MRSA carriage studies among healthy pigs in Portugal are very limited. In total, 101 swine nasal samples from two Portuguese farms were screened for MRSA. In addition five swine workers (including one veterinary and one engineer) and four household members were nasally screened. The isolates were characterized by spa typing, SCCmec typing and MLST. All isolates were tested for antimicrobial susceptibility, presence of mecA and mecC genes, and virulence determinants. MRSA prevalence in swine was 99% (100/101), 80% (4/5) in swine workers and 25% (1/4) in household members. All isolates belonged to ST398 distributed over two spa types–t011 (57%) and t108 (42%). SCCmec type V was present in most of the isolates (n = 95; 82%) while 21 isolates amplified the mecA gene only and were classified as nontypeable. The majority of the isolates were resistant to tetracycline (100%), clindamycin (97%), erythromycin (96%), chloramphenicol (84%) and gentamycin (69%). Notably, 12% showed resistance to quinupristin-dalfopristin (MICs 3–8 μg/mL). Beta-hemolysin (81%) and gamma-hemolysin (74%) were the unique virulence determinants detected. None of the isolates harboured PVL or mecC gene. This study showed a massive occurrence of ST398-MRSA in two independent swine farms, highlighting its establishment among healthy pigs in Portugal.