The mouse Secreted frizzled-related protein 5 gene is expressed in the anterior visceral endoderm and foregut endoderm during early post-implantation development
The mouse Secreted frizzled-related protein 5 gene is expressed in the anterior visceral endoderm and foregut endoderm during early post-implantation development
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DOI：
10.1016/s1567-133x(03)00091-7
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发表时间：
2003-10-01
影响因子：
1.2
通讯作者：
Shawlot, W
中科院分区：
文献类型：
--
作者：
Finley, KR;Tennessen, J;Shawlot, W
前内脏内胚层（AVE）在小鼠前后轴的形成中起重要作用。AVE部分通过表达拮抗近端上胚层中生长因子信号传导的分泌因子来发挥功能。在这里，我们报告的分泌卷曲相关蛋白5（Sfrp5）基因，它编码的分泌因子，可以拮抗Writ信号，在AVE和前肠内胚层在小鼠发育早期表达。在胚胎第5.5天，Sfrp5在胚胎远端区域的内脏内胚层中表达，并且在E6.5在原始条纹对面的AVE中表达。在Lim1胚胎中，缺乏前部神经组织，有时形成次级体轴，Sfrp5表达细胞无法向前部移动，并保留在E6.5胚胎的远端。当与Dkk1（其编码存在于内脏内胚层中的另一种分泌的Wnt拮抗剂分子）相比时，Sfrp5和Dkk1表达重叠，但frp5在AVE中表达更广泛。在E7.5和8之间，Sfrp5在心脏中胚层下面的前肠内胚层中表达。在E8.5，Sfrp5在腹侧前肠内胚层中表达，产生肝脏。Sfrp5表达的其他领域发生在背神经管和前脑前部的视基板。这些研究结果确定了一个基因编码的分泌Wnt拮抗剂，表达在胚胎外内脏内胚层和前定形内胚层在轴形成和器官发生在小鼠。(C)2003 Elsevier B.V.保留所有权利。
The anterior visceral endoderm (AVE) plays an important role in anterior-posterior axis formation in the mouse. The AVE functions in part by expressing secreted factors that antagonize growth factor signaling in the proximal epiblast. Here we report that the Secreted frizzled-related protein 5 (Sfrp5) gene, which encodes a secreted factor that can antagonize Writ signaling, is expressed in the AVE and foregut endoderm during early mouse development. At embryonic day (E) 5.5, Sfrp5 is expressed in the visceral endoderm at the distal tip region of the embryo and at E6.5 in the AVE opposite the primitive streak. In Lim1 embryos, which lack anterior neural tissue and sometimes form a secondary body axis, Sfrp5-expressing cells fail to move towards the anterior and remain at the distal tip of E6.5 embryos. When compared with Dkk1, which encodes another secreted Wnt antagonist molecule present in the visceral endoderm, Sfrp5 and Dkk1 expression overlap but frp5 is expressed more broadly in the AVE. Between E7.5 and 8, Sfrp5 is expressed in the foregut endoderm underlying the cardiac mesoderm. At E8.5, Sfrp5 is expressed in the ventral foregut endoderm that gives rise to the liver. Additional domains of Sfrp5 expression occur in the dorsal neural tube and in the forebrain anterior to the optic placode. These findings identify a gene encoding a secreted Wnt antagonist that is expressed in the extraembryonic visceral endoderm and anterior definitive endoderm during axis formation and organogenesis in the mouse. (C) 2003 Elsevier B.V. All rights reserved.