Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution.
Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution.
复制标题
研究动脉粥样硬化进展和消退中单核细胞和巨噬细胞贩运的方法。
DOI：
10.1007/978-1-4939-9130-3_12
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Fisher,EdwardA
中科院分区：
文献类型：
--
作者：
Weinstock,Ada;Fisher,EdwardA
单核细胞是对病原体的应答至关重要的循环细胞。感染后，它们迅速被招募到受影响的组织，在那里它们可以分化成专门的吞噬细胞和抗原呈递细胞。此外，单核细胞在慢性炎症中起着至关重要的作用，它们可以促进和增强炎症或诱导其消退。单核细胞有两个主要的亚群，“炎性”和“非经典”，它们被认为具有不同的功能。在动脉粥样硬化中，这两种类型的单核细胞不断被募集到病变，在那里它们有助于斑块形成和动脉粥样硬化进展。令人惊讶的是，这些细胞也可以被招募到病变并促进动脉粥样硬化的消退。在不同的疾病阶段跟踪这些细胞可以了解炎症和消退组织中发生的动态变化。在这一章中，我们将讨论的两个单核细胞亚群的差异标记的方法，以检查它们在炎症中的动力学。
Monocytes are circulating cells imperative to the response against pathogens. Upon infection, they are quickly recruited to the affected tissue where they can differentiate into specialized phagocytes and antigen-presenting cells. Additionally, monocytes play a vital role in chronic inflammation, where they can promote and enhance inflammation or induce its resolution. There are two major subsets of monocytes, “inflammatory” and “nonclassical,” which are believed to have distinct functions. In atherosclerosis, both types of monocytes are constantly recruited to lesions, where they contribute to plaque formation and atherosclerosis progression. Surprisingly, these cells can also be recruited to lesions and promote resolution of atherosclerosis. Tracking these cells in various disease stages may inform about the dynamic changes occurring in the inflamed and resolving tissues. In this chapter we will discuss methods for differential labeling of the two monocyte subsets in order to examine their dynamics in inflammation.