CHANGES IN AMINO-ACIDS, ENZYME-ACTIVITIES, AND BIOMASSES DURING SOIL MICROBIAL-GROWTH
CHANGES IN AMINO-ACIDS, ENZYME-ACTIVITIES, AND BIOMASSES DURING SOIL MICROBIAL-GROWTH
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DOI：
10.1097/00010694-197901000-00004
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
PIOVANELLI, C
中科院分区：
文献类型：
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作者：
NANNIPIERI, P;PEDRAZZINI, F;PIOVANELLI, C
研究了红壤中添加葡萄糖和NaNO 3溶液后微生物生长过程中CO2产生、脲酶、磷酸酶和蛋白酶活性、氨基酸、氨基糖、细菌和真菌的行为。CO2释放速率的峰值先于所有其他测试的生化和微生物标准。脲酶和磷酸酶活性与细菌生物量显著相关，但与真菌生物量不相关。所有其他生化指标测试没有显示出相关性，在正常的回归分析，与生物量。真菌生长发生在葡萄糖修正的土壤和控制。在所测试的酶之间观察到的时间序列是由于它们在葡萄糖的土壤代谢中的不同作用，以及它们不同的细胞位置。新合成的酸溶性、氨基酸N和蛋白酶活性都是短暂的。然而，脲酶和磷酸酶活性发生了某种稳定。氨基酸总量的变化主要是由于中性和酸性氨基酸的行为引起的。测试的氨基酸，赖氨酸，组氨酸，精氨酸，缬氨酸，异亮氨酸，亮氨酸，苯丙氨酸显示出可忽略不计的差异值的相应的土壤样品润湿葡萄糖-硝酸盐溶液和蒸馏水。没有一种活性测量方法足以解释土壤系统中微生物的生长。测量的每个指标必须与其他测试结合使用，以全面了解系统。
The behavior of CO2 production, urease, phosphatase and protease activities, amino acids, amino sugars, bacteria and fungi was investigated during the microbial growth after the addition of a glucose NaNO3 solution to a red earth soil. The peak of the CO2 evolution rate preceded all other tested biochemical and microbiological criteria. Urease and phosphatase activities were significantly correlated with bacterial but not with fungal biomass. All other biochemical criteria tested did not show a correlation, on normal regression analysis, with biomass. Fungal growth occurred in the glucose-amended soil and in the control. The temporal sequence observed among the tested enzymes was due to their different roles in the soil metabolism of glucose, as well as to their different cellular locations. The newly synthesized, acid-soluble, amino acids N and protease activity were short-lived. Yet, some sort of stabilization for urease and phosphatase activities occurred. The changes in total amino acids N were due to behaviors or neutral and acidic amino acids. Of the amino acids tested, lysine, histidine, arginine, valine, isoleucine, leucine, and phenylalanine showed negligible differences among values of corresponding soil samples moistened with glucose-nitrate solution and with distilled water. No one measurement of activity is sufficient to interpret microbial growth in the soil system. Each of the indices measured must be used in conjunction with other tests to have a complete picture of the system.