Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus.
Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus.
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DOI：
10.1111/j.1601-0825.2011.01869.x
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发表时间：
2012-04
期刊：
影响因子：
3.8
通讯作者：
W-Y. Zhang;W. Liu;Y-M Zhou;X-M Shen;Y-F Wang;G. Tang
中科院分区：
文献类型：
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作者：
W-Y. Zhang;W. Liu;Y-M Zhou;X-M Shen;Y-F Wang;G. Tang
越来越多的证据表明microRNAs（miRNAs）在炎症和自身免疫性疾病的发病机制中起着重要作用。本研究的目的是研究口腔扁平苔藓（OLP）患者中改变的miRNA表达谱，并确定miR-27 b的表达。方法我们使用微阵列技术比较了OLP患者（n=3）和正常对照（n =3）口腔活检标本中miRNA的表达模式。我们进一步使用实时定量PCR（RT-QPCR）评估了OLP患者（n=53）与对照组（n=34）标本中的miR-27 b表达，并使用原位杂交（ISH）评估了OLP患者（n=15）与对照组（n=12）标本中的miR-27 b表达。结果通过基因芯片分析，共鉴定出46个差异表达超过2倍的miRNAs，其中上调8个，下调38个。RT-QPCR和ISH分析均显示，miR-27 b在OLP组织中显著下调，并且miR-27 b在萎缩糜烂型OLP中的表达比网状OLP中更受抑制。此外，发现miR-27 b在正常和OLP组织的上皮角质形成细胞层中表达。结论miRNAs可能是OLP发病机制中新的候选生物标志物。
BACKGROUND Increasing evidence indicates that microRNAs (miRNAs) play a vital role in the pathogenesis of inflammatory and autoimmune diseases. The objective of this study was to investigate the altered miRNA expression profile in patients with oral lichen planus (OLP) and determine the miR-27b expression. METHODS We compared miRNA expression patterns in oral biopsy specimens from patients with OLP (n=3) with those from normal controls (n=3) using microarray technology. We further assessed the miR-27b expression in specimens from patients with OLP (n=53) against controls (n=34) using real-time quantitative PCR (RT-QPCR), and miR-27b expression in specimens from patients with OLP (n=15) against controls (n=12) using in situ hybridization (ISH). RESULTS Using microarray analysis, a total of 46 differentially expressed miRNAs with more than 2-fold change were identified, including 8 up-regulated and 38 down-regulated miRNAs. Both RT-QPCR and ISH analyses revealed that miR-27b was significantly down-regulated in OLP tissue, and miR-27b expression was even more suppressed in atrophic-erosive OLP than in reticular OLP. In addition, miR-27b was found to be expressed in the epithelial keratinocyte layer of both normal and OLP tissues. CONCLUSION These data indicate that miRNAs may be the novel candidate biomarkers for the implication of miRNAs in the pathogenesis of OLP.