Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel
Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel
复制标题
通过微流体收缩通道表征单核电特性
DOI：
10.3390/mi10110740
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发表时间：
2019-11-01
期刊：
影响因子：
3.4
通讯作者：
Chen, Jian
中科院分区：
文献类型：
--
作者：
Liang, Hongyan;Zhang, Yi;Chen, Jian
作为关键的生物电标志，核膜的等效电容（Cne，即单位面积的电容）和电阻（Rne，即电阻率乘以厚度）已成为有前景的电学指标，而传统方法无法有效测量它们。在这项研究中，从整个细胞中分离出单核，并将其捕获在微流控通道的入口处，然后对相应的阻抗曲线进行采样，并基于自主开发的等效电模型将其转化为单核Cne和Rne。 A549 细胞核的 Cne 和 Rne 首先被量化为 3.43 ± 1.81 μF/cm2 和 2.03 ± 1.40 Ω·cm2 (Nn = 35)，结果表明这不受核分离和测量中关键参数变化的影响。本研究中开发的方法还用于测量第二种类型的原子核，对于 SW620 (Nn = 17)，产生的 Cne 和 Rne 为 3.75 ± 3.17 μF/cm2 和 1.01 ± 0.70 Ω·cm2。这项研究可能为单细胞电学表征提供新的视角，从而实现基于细胞核生物电标记的细胞类型分类和细胞状态评估。
As key bioelectrical markers, equivalent capacitance (Cne, i.e., capacitance per unit area) and resistance (Rne, i.e., resistivity multiply thickness) of nuclear envelopes have emerged as promising electrical indicators, which cannot be effectively measured by conventional approaches. In this study, single nuclei were isolated from whole cells and trapped at the entrances of microfluidic constriction channels, and then corresponding impedance profiles were sampled and translated into single-nucleus Cne and Rne based on a home-developed equivalent electrical model. Cne and Rne of A549 nuclei were first quantified as 3.43 ± 1.81 μF/cm2 and 2.03 ± 1.40 Ω·cm2 (Nn = 35), which were shown not to be affected by variations of key parameters in nuclear isolation and measurement. The developed approach in this study was also used to measure a second type of nuclei, producing Cne and Rne of 3.75 ± 3.17 μF/cm2 and 1.01 ± 0.70 Ω·cm2 for SW620 (Nn = 17). This study may provide a new perspective in single-cell electrical characterization, enabling cell type classification and cell status evaluation based on bioelectrical markers of nuclei.