Human Nasal Mucosa Contains Tissue-Resident Immunologically Responsive Mesenchymal Stromal Cells
Human Nasal Mucosa Contains Tissue-Resident Immunologically Responsive Mesenchymal Stromal Cells
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DOI：
10.1089/scd.2009.0245
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Brandau, Sven
中科院分区：
文献类型：
--
作者：
Jakob, Mark;Hemeda, Hatim;Brandau, Sven
多能间充质基质细胞（Multipotent mesenchymal stromal cells，MSC）广泛存在于骨髓及脂肪组织、肌肉、胰腺、肝脏等组织中，可迁移至感染、炎症和癌症部位，并与不同的免疫细胞亚群相互作用。在这里，我们报告的第一次多能鼻粘膜来源的间充质基质细胞（nm-MSC）的分离和表征。nm-MSC表现出可塑性粘附和成纤维细胞样形态，并能够形成集落。它们表达典型的骨髓MSC标志物抗原CD 29、CD 44、CD 73、CD 90和CD 105，并且能够沿着脂肪形成、软骨形成和成骨途径分化。nm-MSC产生一组炎性细胞因子，表达趋化因子受体，并对细胞因子、趋化因子和TLR 4配体LPS的刺激有反应。因此，这些细胞可以作为再生组织修复的替代成体基质细胞资源，并可能代表局部粘膜免疫的重要调节因子。
Multipotent mesenchymal stromal cells (MSC) are present in bone marrow and other tissues such as adipose tissue, muscle, pancreas, liver, and so on. Recent evidence suggests that MSC migrate to sites of infection, inflammation, and cancer, and interact with different immune cell subsets. Here, we report for the first time on the isolation and characterization of multipotent nasal mucosa-derived mesenchymal stromal cells (nm-MSC). nm-MSC showed a plastic adherent and fibroblast-like morphology and were able to form colonies. They expressed the typical bone marrow MSC marker antigens CD29, CD44, CD73, CD90, and CD105 and were able to differentiate along the adipogenic, chondrogenic, and osteogenic pathways. nm-MSC produced a set of inflammatory cytokines, expressed chemokine receptors, and were responsive to stimulation with cytokines, chemokines, and TLR4 ligand LPS. Thus, these cells may serve as an alternative adult stromal cell resource for regenerative tissue repair and may represent important regulators of local mucosal immunity.