Endogenous plasma proteins in edematous lungs and alveolar fluid in rabbits.
Endogenous plasma proteins in edematous lungs and alveolar fluid in rabbits.
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兔子水肿肺和肺泡液中的内源性血浆蛋白。
DOI：
10.1152/jappl.1985.59.6.1971
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Cheney,FW
中科院分区：
文献类型：
--
作者：
Pavlin,DJ;Haschke,R;Nessly,ML;Cheney,FW
在这项研究中，我们比较了两种方法来区分静水型和渗透型肺水肿。第一种方法需要直接测量肺泡液（AF）样本中的蛋白质浓度;第二种方法是间接技术，其中血管外细胞外水（EVECW）中的蛋白质浓度是根据肺中蛋白质的量和EVECW的体积的单独测量来计算的。在离体水肿兔肺中测定EVECW和肺泡液中白蛋白（Alb）和γ-G-球蛋白的浓度。水肿由左心室舒张末期压升高至25 Torr（静水性水肿，HE）或静脉注射油酸0.09 ml/kg（渗透性水肿，PE）引起。Na+的分布容积被用作肺中EVECW的量度。在两种类型的水肿中，比较EVECW和AF相对于血浆的蛋白浓度（分别为EV/PL和AF/PL）。He组中Alb的EV/PL为0.61 +/- 0.12（SD），而PE组为1.18 +/- 0.47（P <0.02）。HE和PE的AF/PL分别为0.54 +/- 0.12和1.25 +/- 0.33（P <0.001）。白蛋白的EV/PL与AF/PL之间有良好的相关性（r = 0.74，P <0.001），而γ-G-球蛋白的EV/PL与AF/PL之间无相关性。因此，白蛋白的EV/PL、白蛋白的AF/PL和γ-G-球蛋白都能区分静水性水肿和渗透性水肿。
In this study, we compared two methods of differentiating hydrostatic and permeability types of pulmonary edema. The first method entailed measurement of protein concentrations directly in samples of alveolar fluid (AF); the second method was an indirect technique in which protein concentration in extravascular extracellular water (EVECW) was calculated on the basis of separate measurements of the quantity of protein in the lung and the volume of EVECW. The concentration of albumin (Alb) and gamma-G-globulin was measured in EVECW and alveolar fluid in excised edematous rabbit lungs. Edema was caused by elevation of left ventricular end-diastolic pressure to 25 Torr (hydrostatic edema, HE) or by intravenous oleic acid, 0.09 ml/kg (permeability edema, PE). The volume of distribution of Na+ was utilized as a measure of EVECW in the lung. Protein concentration in EVECW and AF relative to plasma (EV/PL and AF/PL, respectively) was compared in the two types of edema. The EV/PL was 0.61 +/- 0.12 (SD) for Alb in He compared with 1.18 +/- 0.47 in PE (P less than 0.02). The AF/PL was 0.54 +/- 0.12 and 1.25 +/- 0.33 in HE and PE, respectively (P less than 0.001). There was good correlation between EV/PL and AF/PL for Alb (r = 0.74, P less than 0.001) but not for gamma-G-globulin. Thus EV/PL for Alb, AF/PL for Alb, and gamma-G-globulin all differentiated hydrostatic from permeability edema.