Number and proliferation of basal and parabasal cells in normal human airway epithelium
Number and proliferation of basal and parabasal cells in normal human airway epithelium
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DOI：
10.1164/ajrccm.157.6.9707011
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发表时间：
1998-06-01
影响因子：
24.7
通讯作者：
Thunnissen, FBJM
中科院分区：
文献类型：
--
作者：
Boers, JE;Ambergen, AW;Thunnissen, FBJM
根据实验动物的研究，基底细胞有两种作用：(1)固定气管支气管上皮；(2)上皮干细胞。位于基底细胞上方的旁基底细胞也被证明有助于细胞更新。然而，缺乏对正常人肺基底细胞和旁基底细胞组成和增殖的系统研究。本研究的目的是确定正常人导气管上皮：(1)基底细胞和旁基底细胞的数量；(2)基底细胞和旁基底细胞对增殖分数的贡献。从尸检获得的7个肺中提取组织学上正常的组织样本，无肺部疾病。免疫组织化学染色采用一抗MIB-1作为增殖标记，抗角蛋白抗体34 β E12作为基底细胞和旁基底细胞的标记。在最大的传导气道（直径大于或等于4mm）中，基底细胞和旁基底细胞的百分比分别为31%和7%；基底细胞和副基底细胞对增殖室的贡献分别为51%和33%。在最小的气道（直径< 0.5 mm）中，6%的上皮细胞为基底细胞，30%的上皮细胞为增殖室，而旁基底细胞不存在。正常人类导气管上皮增生室中基底和旁基底细胞的高比例支持了基底膜或基底膜附近的细胞可能是祖细胞的理论。
Two roles have been suggested for basal cells on the basis of studies performed with laboratory animals: (I) anchoring of the tracheobronchial epithelium; and (2) being the epithelial stem cell. Parabasal cells located just above the basal cells have also been shown to contribute to cell renewal. However, a systematic study of the composition and proliferation of basal and parabasal cells in normal human lungs is lacking. The aims of this study were to determine in normal human conducting-airway epithelium: (1) the number of basal and parabasal cells; and (2) the contribution of basal and parabasal cells to the proliferation fraction. Samples of histologically normal tissue, free of pulmonary disease, were taken from seven lungs obtained by autopsy. Immunohistochemical staining was performed with the primary antibody MIB-1 as a proliferation marker and the antikeratin antibody 34 beta E12 as a marker for basal and parabasal cells. In the largest conducting airways (diameter greater than or equal to 4 mm), the percentages of basal and parabasal cells were 31% and 7%, respectively; the contribution to the proliferation compartment was 51% for basal and 33% for parabasal cells. In the smallest airways (diameter < 0.5 mm), 6% of epithelial cells were basal cells, with a 30% contribution to the proliferation compartment, whereas parabasal cells were absent. The high fraction of basal and parabasal cells contributing to the proliferation compartment of normal human conducting-airway epithelium supports the theory that cells at or near the basement membrane are likely to be progenitor cells.