Epstein-Barr virus BHRF1 functions downstream of Bid cleavage and upstream of mitochondrial dysfunction to inhibit TRAIL-induced apoptosis in BJAB cells
Epstein-Barr virus BHRF1 functions downstream of Bid cleavage and upstream of mitochondrial dysfunction to inhibit TRAIL-induced apoptosis in BJAB cells
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DOI：
10.1016/s0006-291x(02)02261-1
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发表时间：
2002-09-27
影响因子：
3.1
通讯作者：
Kawanishi, S
中科院分区：
文献类型：
--
作者：
Kawanishi, M;Tada-Oikawa, S;Kawanishi, S
我们先前已经报道，肿瘤坏死因子相关的凋亡诱导配体(TRAIL)通过激活caspase-8和线粒体膜电位(DeltaPsim)而导致Bid的裂解，从而导致细胞凋亡。表达EB病毒(EBV)抗凋亡蛋白BHRF1的BJAB克隆实验表明，BHRF1可显著抑制TRAIL介导的细胞凋亡。虽然Western印迹分析表明BHRF1不能抑制TRAIL诱导的BID裂解，但BHRF1能有效地阻断TRAIL诱导的DeltaPsim的下降。这些结果表明，在BJAB细胞中，BHRF1作用于BID裂解的下游和线粒体损伤的上游，从而抑制TRAIL诱导的细胞凋亡。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
We have previously reported that TNF-related apoptosis inducing ligand (TRAIL) causes cleavage of Bid via activation of caspase-8 and the loss of mitochondrial membrane potential (DeltaPsim), resulting in apoptosis. Experiments with BJAB clones expressing Epstein-Barr virus (EBV) anti-apoptotic protein BHRF1 showed that BHRF1 drastically inhibited TRAIL-mediated apoptosis. Although Western blot analysis demonstrated that TRAIL-induced Bid cleavage was not inhibited by BHRF1, the decrease in DeltaPsim caused by TRAIL was effectively blocked by BHRF1 These findings suggest that in BJAB cells, BHRF1 acts downstream of Bid cleavage and upstream of mitochondrial damage, resulting in inhibition of TRAIL-induced apoptosis. (C) 2002 Elsevier Science (USA). All rights reserved.