Polyphenol Oxidase Activity in Subcellular Fractions of Tall Fescue Contaminated by Polycyclic Aromatic Hydrocarbons
Polyphenol Oxidase Activity in Subcellular Fractions of Tall Fescue Contaminated by Polycyclic Aromatic Hydrocarbons
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多环芳烃污染的高羊茅亚细胞组分中的多酚氧化酶活性
DOI：
10.2134/jeq2011.0461
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发表时间：
2012-05-01
影响因子：
2.4
通讯作者：
Sun, Yandi
中科院分区：
文献类型：
--
作者：
Ling, Wanting;Lu, Xiaodan;Sun, Yandi
了解酶对持久性有机污染物污染的响应是阐明植物持久性有机污染物代谢机制的关键步骤。然而，关于POP污染植物亚细胞组分中的酶活性的信息很少。据我们所知，这是首次利用温室批处理技术研究多环芳烃(PAHs)污染胁迫下植物细胞组分中多酚氧化酶(PPO)的活性。E-cell、Ecell-n和P-cell三个参数分别表示PPO活性的数量、细胞组分含量、归一化PPO活性以及PPO活性在各细胞组分中的比例。高羊茅(Festuca arundinacea Schreb.)培养液中较高浓度的菲(>；0.23 mg L~(-1))对高羊茅PPO活性有明显的促进作用。根和枝以及它们的细胞部分。菲污染根和地上部各细胞组分中PPO活性的数量和分布比例从大到小依次为：细胞液、细胞壁和细胞器。细胞液是PPO活性的主要储存区，对根和地上部PPO活性的贡献率分别为84.0%和82.8%。根和地上部的PPO活性密度最高的是细胞壁，归一化的PPO活性在该细胞部分的含量最高。我们的结果提供了有关植物细胞内各组分对异种POPs的酶反应的新信息，以及植物内POP代谢机制的基本信息。
Understanding enzyme responses to contamination with persistent organic pollutants (POPs) is a key step in the elucidation of POP metabolic mechanisms in plants. However, there is little information available on enzyme activity in subcellular fractions of POP-contaminated plants. To our knowledge, this is the first study to investigate the activities of polyphenol oxidase (PPO) in cell fractions of plants under contamination stress from polycyclic aromatic hydrocarbons (PAHs) using a greenhouse batch technique. Three parameters, E-cell, Ecell-n, and P-cell, denoting the amount of PPO activity, cell fraction content normalized PPO activity, and proportion of PPO activity in each cell fraction, respectively, were used in this study. Contamination with phenanthrene, as a representative PAH, at a relatively high level (>0.23 mg L-1) in culture solution generally stimulated PPO activity in tall fescue (Festuca arundinacea Schreb.) roots and shoots and their cellular fractions. The amount and distribution proportion of PPO activity in each cell fraction of phenanthrene-contaminated roots and shoots were (in descending order): cell solution > > cell wall > cell organdies. Cell solution was the dominant storage domain of PPO activity and contributed 84.0 and 82.8% of PPO activity in roots and shoots, respectively. The cell wall had the highest density of PPO activity in roots and shoots, based on the highest cell fraction content normalized PPO activity in this cell fraction. Our results provide new information on enzyme responses in plant intracellular fractions to xenobiotic POPs and fundamental information on within-plant POP metabolic mechanisms.