A Novel Method for Transmission Electron Microscopy Study of Cytoplasmic Fragments From Preimplantation Human Embryos
A Novel Method for Transmission Electron Microscopy Study of Cytoplasmic Fragments From Preimplantation Human Embryos
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DOI：
10.1002/jemt.22657
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发表时间：
2016-06-01
影响因子：
2.5
通讯作者：
Nottola, Stefania A.
中科院分区：
文献类型：
--
作者：
Halvaei, Iman;Khalili, Mohammad Ali;Nottola, Stefania A.
透射电子显微镜是研究细胞内损伤和细胞器分布的主要工具。产生胚胎胞质碎裂的原因尚不完全清楚。由于碎片对胚胎发育有不利影响，因此碎片的超微结构分析可能在碎片病因学和胚胎发育中发挥重要作用。目前还没有关于可移植胚胎中碎片超微结构的研究，因为透射电子显微镜的制备并不重要，胚胎不可避免地会被丢弃。本研究旨在介绍一种在不损伤人类卵裂胚胎的情况下对碎片进行超微结构评价的新方法。(C)2016威利期刊公司。
Transmission electron microscopy (TEM) is the main tool for exploring the intracellular damage and organelle distribution. The cause of producing embryo cytoplsamic fragmentation is not completely understood. Since the fragments have detrimental effects on embryo development, the ultrastructural analysis of fragments may play an important role in fragmentation etiology and in embryo development as well. There are no studies regarding the ultrastructure of fragments in transferable embryos, because the preparation for TEM is not vital and embryos are discarded inevitably. This study aims to introduce a new method for ultrastructural evaluation of fragments without damaging the human cleaving embryos. (C) 2016 Wiley Periodicals, Inc.