Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states
Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states
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DOI：
10.1101/gad.221648.113
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发表时间：
2013-07-15
影响因子：
10.5
通讯作者：
Pikaard, Craig S.
中科院分区：
文献类型：
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作者：
Pontvianne, Frederic;Blevins, Todd;Pikaard, Craig S.
真核生物具有数千个45S核糖体RNA （rRNA）基因，其中许多基因在发育过程中被沉默。利用荧光激活分选技术，我们发现拟南芥中有活性的rRNA基因存在于分选的核仁中，而沉默的rRNA基因则被排除在外。DNA甲基转移酶（met1），组蛋白去乙酰化酶（hda6），或染色质组装（caf1）突变破坏沉默，取消这种核质-核仁分配。亚硫酸氢盐测序数据表明，活性核仁rRNA基因在启动子cg上几乎完全去甲基化，而沉默基因几乎完全甲基化。总的来说，这些数据揭示了rRNA基因根据其表观遗传状态占据不同但可变的核区域。
Eukaryotes can have thousands of 45S ribosomal RNA ( rRNA) genes, many of which are silenced during development. Using fluorescence-activated sorting techniques, we show that active rRNA genes in Arabidopsis thaliana are present within sorted nucleoli, whereas silenced rRNA genes are excluded. DNA methyltransferase (met1), histone deacetylase (hda6), or chromatin assembly (caf1) mutants that disrupt silencing abrogate this nucleoplasmic-nucleolar partitioning. Bisulfite sequencing data indicate that active nucleolar rRNA genes are nearly completely demethylated at promoter CGs, whereas silenced genes are nearly fully methylated. Collectively, the data reveal that rRNA genes occupy distinct but changeable nuclear territories according to their epigenetic state.