Ion-ion proton transfer reactions of multiply-charged oligonucleotide cations
Ion-ion proton transfer reactions of multiply-charged oligonucleotide cations
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DOI：
10.1016/s0168-1176(97)00176-6
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发表时间：
1997-11-01
影响因子：
1.8
通讯作者：
McLuckey, SA
中科院分区：
文献类型：
--
作者：
Stephenson, JL;McLuckey, SA
在四极离子阱中，使来自寡核苷酸5 ′-d（CT-TAGCGCTAAG）-3 ′的正离子电喷雾的多电荷阳离子与来自全氟-1，3-二甲基环己烷的（M-CF 3）（-）和（M-F）（-）阴离子反应。研究了自互补寡核苷酸形成的单链离子和双链离子。与多质子化蛋白质与相同阴离子的反应类似，未观察到加合物形成，数据与排他性质子转移一致。对于单链离子或双链离子，均未观察到断裂证据，尽管仅根据这些结果无法排除后一种物质的小程度断裂。与寡核苷酸阳离子与衍生自PDCH的阴离子的离子-离子反应相关的简单且定义明确的化学对于中等大小的寡聚物（至多约100个残基）的电荷状态操纵是期望的，因为PDCH阴离子具有用于产生和储存具有数万个质荷比的离子/离子反应产物的许多期望特性。(C)1997年Elsevier Science B.V.
Multiply-charged cations derived from the positive ion electrospray of the oligonucleotide 5'-d(CT-TAGCGCTAAG)-3' were subjected to reactions with the (M-CF3)(-) and (M-F)(-) anions derived from perfluoro-1,3-dimethylcyclohexane in a quadrupole ion trap. Both single strand ions and double strand ions formed from the self-complementary oligonucleotide were studied. In analogy with the reactions of multiply-protonated proteins with the same anions, no adduct formation was observed and the data were consistent with exclusive proton transfer. No evidence for fragmentation was observed for either the single strand ions or the double strand ions, although a small degree of fragmentation of the latter species could not be precluded based on these results alone. The simple and well-defined chemistry associated with the ion-ion reactions of oligonucleotide cations with anions derived from PDCH is desirable for the charge-state manipulation of moderately-sized oligomers (up to roughly 100 residues) because PDCH anions possess a number of desirable characteristics for the production and storage of ion/ion reaction products with mass-to-charge ratios in the tens of thousands. (C) 1997 Elsevier Science B.V.