Spontaneous conversion of nontransformed avian sarcoma virus-infected rat cells to the transformed phenotype.
Spontaneous conversion of nontransformed avian sarcoma virus-infected rat cells to the transformed phenotype.
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未转化的禽肉瘤病毒感染的大鼠细胞自发转化为转化的表型。
DOI：
10.1128/jvi.35.2.466-478.1980
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发表时间：
1980
影响因子：
5.4
通讯作者：
Oppermann,H
中科院分区：
文献类型：
--
作者：
Turek,LP;Oppermann,H
用Schmidt-Ruppin株（SR-D）的禽肉瘤病毒（ASV）感染正常大鼠肾（NRK）成纤维细胞，并在感染后20 h克隆，而不选择转化的表型。大多数感染的克隆最初表现出平坦的，非转化的形态，这是未感染的NRK细胞的特征。然而，在长期培养中，大多数SR-D NRK克隆开始分离典型的ASV转化细胞。转化的ASV可以通过与来自大多数测试的感染克隆的鸡胚成纤维细胞融合来拯救。在感染后8至10周通过亚克隆进一步分析三个主要平坦的、独立感染的克隆。从这些“亲本”SR-D NRK克隆系中的两个随机衍生的大多数扁平子代亚克隆在与鸡胚成纤维细胞融合后不产生病毒，尽管从亲本克隆中反复回收了无缺陷的转化ASV。这一观察结果表明，这些SR-D NRK克隆中的大多数（但不是全部）子细胞在克隆后丢失了ASV原病毒。第三个独立亲本细胞克隆c17的后代产生携带ASV的扁平亚克隆和转化亚克隆。在这种情况下，无论细胞的转化表型如何，通过融合和转染从子代亚克隆中回收ASV都是同样有效的。然而，ASVsrc基因的60，000-道尔顿磷蛋白产物仅在转化变体中高水平表达。Luria-Delbruck波动分析和Newcombe再扩散试验的结果表明，导致自发转化为转化状态的事件以预测体细胞突变的速率随机发生在这些扁平ASV NRK细胞的分裂培养物中。
Normal rat kidney (NRK) fibroblasts were infected with the Schmidt-Ruppin strain (SR-D) of avian sarcoma virus (ASV) and cloned 20 h after infection without selection for the transformed phenotype. Most infected clones initially exhibited the flat, nontransformed morphology that is characteristic of uninfected NRK cells. In long-term culture, however, the majority of the SR-D NRK clones began segregating typical ASV-transformed cells. Transforming ASV could be rescued by fusion with chicken embryo fibroblasts from most of the infected clones tested. Three predominantly flat, independently infected clones were further analyzed by subcloning 8 to 10 weeks after infection. Most flat progeny subclones derived at random from two of these “parental” SR-D NRK clonal lines did not yield virus upon fusion with chicken embryo fibroblasts, although a nondefective transforming ASV was repeatedly recovered from the parental clones. This observation suggested that most, but not all, daughter cells in these SR-D NRK clones lost the ASV provirus after cloning. The progeny of the third independent parental cell clone, c17, gave rise to both flat and transformed subclones that carried ASV. In this case, ASV recovery by fusion and transfection from the progeny subclones was equally efficient regardless of the transformation phenotype of the cells. The 60,000-dalton phosphoprotein product of the ASVsrcgene was, however, expressed at high level only in the transformed variants. The results of a Luria-Delbruck fluctuation analysis and of Newcombe's respreading test indicated that the event leading to the spontaneous conversion to the transformed state occurred at random in dividing cultures of these flat ASV NRK cells at a rate predicted for somatic mutation.
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DOI：
10.1016/s0021-9258(19)75875-x
发表时间：
1987-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Roger A. SundeS;Jacqueline K. Evenson
通讯作者：
Roger A. SundeS;Jacqueline K. Evenson
DOI：
10.1016/s0021-9258(18)63520-3
发表时间：
1969-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Burgess
通讯作者：
R. Burgess
影响因子：
4.2
作者：
CHOW, CK;TAPPEL, AL
通讯作者：
TAPPEL, AL
硒作为从绵羊红细胞中分离出的谷胱甘肽过碘酶的成分。
影响因子：
2.9
作者：
S. Oh;H. Ganther;W. Hoekstra
通讯作者：
W. Hoekstra
DOI：
10.1016/s0021-9258(18)62682-1
发表时间：
1970
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Stossel;F. Murad;R. Mason;M. Vaughan
通讯作者：
M. Vaughan