Detection of novel blast resistance genes, Pi58(t) and Pi59(t), in a Myanmar rice landrace based on a standard differential system
Detection of novel blast resistance genes, Pi58(t) and Pi59(t), in a Myanmar rice landrace based on a standard differential system
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DOI：
10.1007/s11032-013-9865-5
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发表时间：
2013-08-01
影响因子：
3.1
通讯作者：
Kobayashi, Nobuya
中科院分区：
文献类型：
--
作者：
Koide, Yohei;Telebanco-Yanoria, Mary Jeanie;Kobayashi, Nobuya
利用广谱抗病基因是获得水稻稻瘟病持久抗性的有效途径。我们先前调查了948个水稻品种的稻瘟病抗性多样性，发现缅甸水稻地方品种Haoru（国际水稻研究所基因库编号IRGC 33090）对标准差异稻瘟病分离物具有广谱抗性。利用标准菌株和鉴别品种组成的标准稻瘟病鉴别体系，对好乳的广谱抗病性进行了遗传基础研究。为了进行遗传分析，我们使用了一个BC 1F 1群体和BC 1F 2系，它们来自于Haoru与感病品种US-2的杂交。共分离分析表明，好茹的BC 1F 1群体中含有3个R基因。通过群体分离和连锁分析，我们将其中的两个R基因定位在第12和第6染色体上，分别命名为Pi 58（t）和Pi 59（t）。利用标准的差异系统将Pi 58（t）和Pi 59（t）与其他已报道的R基因区分开来。Pi 58（t）的抗性谱与好乳的抗性谱一致，表明Pi 58（t）是好乳广谱抗性的主要基因。此外，Pi 59（t）和第3个基因也被证明是研究和改良水稻抗稻瘟病性的新的有用遗传资源。
The use of broad-spectrum R genes is an effective way to achieve durable resistance against rice blast (Magnaporthe oryzae Couch, anamorph: Pyricularia oryzae Cavara) in rice (Oryza sativa L.). We previously surveyed the diversity of blast resistance in 948 rice varieties and found a Myanmar rice landrace, Haoru (International Rice Research Institute genebank acc. no. IRGC33090), with broad-spectrum resistance against the standard differential blast isolates. Here, we examined the genetic basis of Haoru's broad-spectrum resistance by using the standard blast differential system consisting of the standard isolates and differential varieties. For genetic analysis, we used a BC1F1 population and BC1F2 lines derived from crosses of Haoru with a susceptible variety, US-2. Co-segregation analysis of the reaction pattern in the BC1F1 population against the 20 standard isolates suggested that Haoru harbors three R genes. By using bulk-segregant and linkage analysis, we mapped two of the three R genes on chromosomes 12 and 6, and designated them as Pi58(t) and Pi59(t), respectively. Pi58(t) and Pi59(t) were differentiated from other reported R genes using the standard differential system. The estimated resistance spectrum of Pi58(t) corresponded with that of Haoru, suggesting that Pi58(t) is primarily responsible for Haoru's broad-spectrum resistance. In addition, Pi59(t) and the third gene were also proven to be new and useful genetic resources for studying and improving blast resistance in rice.