DddY, a periplasmic dimethylsulfoniopropionate lyase found in taxonomically diverse species of Proteobacteria
DddY, a periplasmic dimethylsulfoniopropionate lyase found in taxonomically diverse species of Proteobacteria
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DOI：
10.1038/ismej.2010.203
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发表时间：
2011-07-01
期刊：
影响因子：
11
通讯作者：
Johnston, Andrew W. B.
中科院分区：
文献类型：
--
作者：
Curson, Andrew R. J.;Sullivan, Matthew J.;Johnston, Andrew W. B.
大量相容的溶质二甲基磺丙酸盐（DMSP）是由多种海藻合成的。不同的海洋细菌通过不同的机制分解DMSP，其中一些释放出对环境重要的气体二甲基硫化物（DMS）。我们描述了一种酶，DddY，它将DMSP裂解成DMS +丙烯酸酯，位于细菌的周质中，不像其他DMSP裂解酶催化这一反应。在Alcaligenes， Arcobacter和Shewanella菌株中，分别在-，epsilon和-proteobacteria中存在类似dddy的基因。在Alcaligenes中，dddY位于一组ddd和acu基因中，这些基因与其他分解DMSP和丙烯酸酯的细菌相似，但也有显著差异。虽然DMS的产生和Alcaligenes dddY的转录显然都可以通过DMSP细胞的预生长来诱导，但这种底物必须被分解代谢形成丙烯酸酯，这是一种真正的共诱导剂。学报（自然科学版），2011 (5),1191-1200；doi: 10.1038 / ismej.2010.203;主题类别：地球微生物学和微生物对地球化学循环的贡献
The abundant compatible solute dimethylsulfoniopropionate (DMSP) is made by many marine algae. Different marine bacteria catabolise DMSP by various mechanisms, some of which liberate the environmentally important gas dimethyl sulfide (DMS). We describe an enzyme, DddY, which cleaves DMSP into DMS plus acrylate and is located in the bacterial periplasm, unlike other DMSP lyases that catalyse this reaction. There are dddY-like genes in strains of Alcaligenes, Arcobacter and Shewanella, in the beta-, epsilon- and gamma-proteobacteria, respectively. In Alcaligenes, dddY is in a cluster of ddd and acu genes that resemble, but also have significant differences to, those in other bacteria that catabolise both DMSP and acrylate. Although production of DMS and transcription of Alcaligenes dddY are both apparently inducible by pre-growth of cells with DMSP, this substrate must be catabolised to form acrylate, the bona fide coinducer. The ISME Journal (2011) 5, 1191-1200; doi:10.1038/ismej.2010.203; published online 20 January 2011 Subject Category: geomicrobiology and microbial contributions to geochemical cycles