Quantification of Ultraviolet-B Stress-Induced Changes in Nuclear and Cellular Sizes of Tobacco Bright Yellow-2 Cells
Quantification of Ultraviolet-B Stress-Induced Changes in Nuclear and Cellular Sizes of Tobacco Bright Yellow-2 Cells
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DOI：
10.1508/cytologia.84.347
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发表时间：
2019-12
期刊：
影响因子：
1
通讯作者：
Shinya Takahashi;Kei H Kojo;S. Hasezawa
中科院分区：
文献类型：
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作者：
Shinya Takahashi;Kei H Kojo;S. Hasezawa
在高等植物中，紫外线（UV）-B辐射诱导DNA损伤并抑制DNA复制，这随后抑制植物生长。然而，植物细胞对UV-B胁迫的特异性反应在很大程度上仍然未知。此前，我们使用烟草亮黄-2（BY-2）细胞评价系统发现，低剂量的UV-B辐射会诱导培养植物细胞和细胞核的形态变化。在这项研究中，我们定量的艾德UV-B胁迫对转基因BY-2细胞的影响，通过可视化细胞核中的活细胞使用荧光蛋白标记。UV-B照射后1-4天，细胞和细胞核增大。我们还观察到照射后1-2天细胞核和细胞的大小发生了显著变化，分别增加了131.36%和138.42%。在UV-B照射的细胞核大小的比例是类似的细胞周期的S和G2期的细胞核大小的比例。我们认为，低剂量的UV-B通过细胞周期阻滞诱导细胞核和细胞体积的适度增加。
Summary In higher plants, ultraviolet (UV)-B irradiation induces DNA damage and inhibits DNA replication, and this subsequently inhibits plant growth. However, the specific responses of plant cells to UV-B stress remain largely unknown. Previously, we found that low-doses of UV-B irradiation-induced morphological changes in cultured plant cells and nuclei, using an evaluation system with tobacco Bright Yellow-2 (BY-2) cells. In this study, we quantified the effects of UV-B stress on transgenic BY-2 cells, by visualizing the nuclei in living cells using a fluorescent protein marker. The UV-B irradiated cells and their nuclei were enlarged 1–4 days after irra diation. We also observed significant size changes in the nuclei and cells 1–2 days after irradiation, with rates of increase of 131.36 and 138.42%, respectively. The ratio of nuclear sizes in the UV-B–irradiated cells was similar to the ratio of nuclear sizes found in the S and G 2 phases of the cell cycle. We suggest that low-doses of UV-B induce moderate increases of the nuclei and cell volumes through cell cycle arrest.