Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus
Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus
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DOI：
10.1101/gad.183500
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发表时间：
2000-12-01
影响因子：
10.5
通讯作者：
Schibler, U
中科院分区：
文献类型：
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作者：
Damiola, F;Le Minh, N;Schibler, U
在哺乳动物中，昼夜节律振荡器不仅存在于容纳中央起搏器的视交叉上核中，而且存在于大多数外周组织中。据信，SCN时钟通过化学线索（如有节奏地分泌的激素）来携带外周时钟的相位。在这里，我们表明，在光暗或暗-暗条件下的时间喂养限制可以改变外周细胞类型的昼夜节律基因表达的阶段长达12小时，同时离开SCN中的周期基因表达的阶段不受影响。因此，代谢的变化可导致外周振荡器与中枢起搏器的解偶联。进食时间的突然大的变化，类似于光周期的突然变化，逐渐重置有节奏的基因表达的阶段，因此可能通过时钟依赖机制起作用。食物诱导的相位重置在肝脏中比在肾脏、心脏或胰腺中进行得更快，但在白天进食1周后，在所有检查的外周组织中，昼夜节律基因表达的相位是相似的。
In mammals, circadian oscillators exist not only in the suprachiasmatic nucleus, which harbors the central pacemaker, but also in most peripheral tissues. It is believed that the SCN clock entrains the phase of peripheral clocks via chemical cues, such as rhythmically secreted hormones. Here we show that temporal feeding restriction under light-dark or dark-dark conditions can change the phase of circadian gene expression in peripheral cell types by up to 12 h while leaving the phase of cyclic gene expression in the SCN unaffected. Hence, changes in metabolism can lead to an uncoupling of peripheral oscillators from the central pacemaker. Sudden large changes in feeding time, similar to abrupt changes in the photoperiod, reset the phase of rhythmic gene expression gradually and are thus likely to act through a clock-dependent mechanism. food-induced phase resetting proceeds faster in liver than in kidney, heart, or pancreas, but after 1 wk of daytime feeding, the phases of circadian gene expression are similar in all examined peripheral tissues.