Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami
Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami
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DOI：
10.1021/nn2050483
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发表时间：
2012-04-01
期刊：
影响因子：
17.1
通讯作者：
Tinnefeld, Philip
中科院分区：
文献类型：
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作者：
Acuna, Guillermo P.;Bucher, Martina;Tinnefeld, Philip
我们研究了由于金属纳米粒子在荧光团附近的距离依赖性荧光猝灭。在我们的单分子测量中，我们通过使用自组装的DNA结构（DNA折纸）作为试验板，其中荧光团和10 nm金属纳米颗粒都以纳米精度定位，实现了对结构和化学计量的出色控制。这里采用的单分子光谱方法报告的颗粒和染料的共定位，而荧光寿命成像是用来直接获得不同的颗粒的距离的强度和荧光寿命的相关性。我们的数据可以通过精确计算（包括偶极-偶极方向和距离）得到很好的解释。与纳米表面能量转移的更实用的模型拟合产生10.4 nm作为50%能量转移的特征距离。DNA纳米技术的使用与最小的样品使用一起，通过将颗粒直接附着到显微镜盖玻片上的DNA折纸，为利用染料-纳米颗粒相互作用的更复杂的实验铺平了道路。
We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity of a fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as a breadboard where both the fluorophore and the 10 run metallic nanoparticle are positioned with nanometer precision. The single-molecule spectroscopy method employed here reports on the co-localization of particle and dye, while fluorescence lifetime imaging is used to directly obtain the correlation of intensity and fluorescence lifetime for varying particle to de distances. Our data can be well explained by exact calculations that include dipole-dipole orientation and distances. Fitting with a more practical model for nanosurface energy transfer yields 10.4 nm as the characteristic distance of 50% energy transfer. The use of DNA nanotechnology together with minimal sample usage by attaching the particles to the DNA origami directly on the microscope coverslip paves the way for more complex experiments exploiting dye-nanoparticle interactions.