Overexpression of Calreticulin in Malignant and Benign Breast Tumors: Relationship with Humoral Immunity
Overexpression of Calreticulin in Malignant and Benign Breast Tumors: Relationship with Humoral Immunity
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DOI：
10.1159/000335267
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发表时间：
2012-01-01
期刊：
影响因子：
3.5
通讯作者：
Juranic, Zorica
中科院分区：
文献类型：
--
作者：
Eric-Nikolic, Aleksandra;Milovanovic, Zorka;Juranic, Zorica
目的：钙网蛋白是一种多室蛋白，调节许多重要的细胞反应。本研究的目的是阐明肿瘤细胞中钙网蛋白过度表达的强度和位置是否与良性或恶性乳腺疾病患者对钙网蛋白的体液免疫升高有关。方法：本研究涉及手术切除前的27例良性乳腺肿瘤患者和58例恶性乳腺肿瘤患者以及38名健康志愿者。使用免疫组织化学测定石蜡包埋组织中恶性或良性细胞的细胞质或膜钙网蛋白过度表达。采用ELISA法检测血清抗钙网蛋白自身抗体水平。结果：对照者与乳腺肿瘤患者、对照者与非恶性乳腺疾病患者血清中抗钙网蛋白抗体IgA水平存在统计学差异，但血清抗钙网蛋白抗体IgG水平无统计学差异。针对细胞质和共定位膜钙网蛋白产生的针对钙网蛋白的体液免疫与其过度表达的强度不相关，并且即使在其膜定位不存在的情况下也存在。结论：小叶性乳腺癌中钙网蛋白过表达程度低于导管性乳腺癌。与抗钙网蛋白 IgG 抗体相比，局部淋巴结转移患者中抗钙网蛋白 IgA 抗体浓度升高的情况更为频繁。版权所有 (C) 2012 S. Karger AG，巴塞尔
Objective: Calreticulin is a multicompartmental protein which regulates many important cellular responses. The aim of this study was to elucidate whether the intensity and location of calreticulin overexpression in tumor cells are related to the elevated humoral immunity to calreticulin in patients with benign or malignant breast disease. Methods: This study involved 27 patients with benign and 58 patients with malignant breast tumors before surgical resection and 38 healthy volunteers. Cytoplasmatic or membranous calreticulin overexpression in malignant or benign cells in paraffin-embedded tissues was determined using immunohistochemistry. Levels of the serum anti-calreticulin autoantibodies were detected by ELISA. Results: Statistically significant differences between serum levels of IgA of anti-calreticulin antibodies in controls and patients with breast tumors, and between controls and patients with nonmalignant breast diseases were found, but no statistically significant differences were found between levels of serum IgG anti-calreticulin antibodies. Humoral immunity to calreticulin developed against cytoplasmatic and co-localized membranous calreticulin was not correlated to the intensity of its overexpression and was present even in the absence of its membranous localization. Conclusions: The degree of calreticulin overexpression in lobular breast carcinoma is lower than in ductal breast carcinoma. Elevated concentrations of anti-calreticulin IgA antibodies were present more frequently in patients with metastasis in locoregional lymph nodes in comparison to anti-calreticulin IgG antibodies. Copyright (C) 2012 S. Karger AG, Basel