Estrogen receptor-α expression in nociceptive-responsive neurons in the medullary dorsal horn of the female rat
Estrogen receptor-α expression in nociceptive-responsive neurons in the medullary dorsal horn of the female rat
复制标题
DOI：
10.1016/j.ejpain.2009.05.008
复制
发表时间：
2010-03-01
影响因子：
3.6
通讯作者：
Blomqvist, Anders
中科院分区：
文献类型：
--
作者：
Amandusson, Asa;Blomqvist, Anders
雌激素对伤害性刺激的传递和对疼痛障碍的易感性产生实质性影响，这在动物和人类受试者的研究中得到证实。雌激素受体（ER）似乎是至关重要的细胞机制的影响。然而，它还没有澄清是否伤害性神经元激活的疼痛表达ER。在这项研究中，有害的注射福尔马林到雌性大鼠的下唇，从而激活三叉神经尾侧亚核的伤害性神经元所示的免疫组化标记的Fos。使用双标记免疫组织化学方案，在相同切片中观察含ER α的细胞。在脊髓背角浅层，12%的ER α标记细胞，主要位于第二层，也表达了伤害诱导的Fos。这些研究结果表明，伤害性反应神经元在延髓背角表达ER α，从而提供了一个可能的形态学基础的假设，雌激素直接调节疼痛的传输在这个水平。(C)2009年，国际疼痛分会研究协会欧洲联合会。由爱思唯尔有限公司出版。保留所有权利。
Estrogens exert a substantial influence on the transmission of nociceptive stimuli and the susceptibility to pain disorders as made evident by studies in both animals and human subjects. The estrogen receptor ( ER) seems to be of crucial importance to the cellular mechanisms underlying such an influence. However, it has not been clarified whether nociceptive neurons activated by pain express ERs. In this study, a noxious injection of formalin was given into the lower lip of female rats, thereby activating nociceptive neurons in the trigeminal subnucleus caudalis as demonstrated by immunohistochemical labeling of Fos. Using a dual-label immunohistochemistry protocol ER alpha-containing cells were visualized in the same sections. In the superficial layers of the medullary dorsal horn, 12% of ER alpha-labeled cells, mainly located in lamina II, also expressed noxious-induced Fos. These findings show that nociceptive-responsive neurons in the medullary dorsal horn express ER alpha, thus providing a possible morphological basis for the hypothesis that estrogens directly regulate pain transmission at this level. (C) 2009 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved.