GRINtrode: a neural implant for simultaneous two-photon imaging and extracellular electrophysiology in freely moving animals.
GRINtrode: a neural implant for simultaneous two-photon imaging and extracellular electrophysiology in freely moving animals.
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DOI：
10.1117/1.nph.9.4.045009
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发表时间：
2022-10
期刊：
影响因子：
5.3
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中科院分区：
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体内成像和电生理学是探索神经元功能的有力工具，各自提供独特的互补信息，具有优势和局限性。从自由移动的动物的同一神经群体中捕获这两种数据类型将使研究人员能够利用这两种模式的能力，并进一步了解它们如何相互关联。在这里，我们提出了一种头戴式神经植入物，适用于在体内双光子成像的神经元活动，同时在头部固定或纤维耦合自由移动的动物细胞外电记录。一种基于梯度折射率（GRIN）透镜的头戴式神经植入物，其细胞外电记录由GRIN透镜外围的四极提供。神经植入物的设计允许记录头部固定的动物，以及通过将成像系统耦合到相干成像光纤束来自由移动的动物。我们证明了同时双光子成像GCaMP和细胞外的神经活动的电生理学在清醒的头部固定和自由移动的小鼠。利用收集到的信息，我们进行相关性分析，揭示光学和局部场电位记录之间的正相关性。使用光学和电学方法同时记录神经活动提供了来自每种模态的互补信息。可以在自由运动的动物中提供这种双模式记录的设计允许研究更广泛的行为范式背后的神经活动。
In vivo imaging and electrophysiology are powerful tools to explore neuronal function that each offer unique complementary information with advantages and limitations. Capturing both data types from the same neural population in the freely moving animal would allow researchers to take advantage of the capabilities of both modalities and further understand how they relate to each other. Here, we present a head-mounted neural implant suitable for in vivo two-photon imaging of neuronal activity with simultaneous extracellular electrical recording in head-fixed or fiber-coupled freely moving animals. A gradient refractive index (GRIN) lens-based head-mounted neural implant with extracellular electrical recording provided by tetrodes on the periphery of the GRIN lens was chronically implanted. The design of the neural implant allows for recording from head-fixed animals, as well as freely moving animals by coupling the imaging system to a coherent imaging fiber bundle. We demonstrate simultaneous two-photon imaging of GCaMP and extracellular electrophysiology of neural activity in awake head-fixed and freely moving mice. Using the collected information, we perform correlation analysis to reveal positive correlation between optical and local field potential recordings. Simultaneously recording neural activity using both optical and electrical methods provides complementary information from each modality. Designs that can provide such bi-modal recording in freely moving animals allow for the investigation of neural activity underlying a broader range of behavioral paradigms.