ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis elegans
ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis elegans
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DOI：
10.1242/dev.01638
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发表时间：
2005-02-01
期刊：
影响因子：
4.6
通讯作者：
Baumeister, R
中科院分区：
文献类型：
--
作者：
Cassata, G;Shemer, G;Baumeister, R
engrailed 是一种同源盒基因，对于节肢动物和脊椎动物的细胞群分化和区室边界的出现等发育功能至关重要。我们提出了第一个针对未分节动物：线虫秀丽隐杆线虫的功能研究。在发育中的蠕虫胚胎中，ceh-16/engrailed 主要在两侧一排表皮细胞（接缝细胞）中表达。我们表明，ceh-16/engrailed 通过三种机制引发规范级联：（1）它通过抑制 eff-1/融合原的表达来抑制接缝细胞与其他表皮细胞之间的融合； (2)通过不同因子触发seam细胞的分化，包括GATA因子elt-5； (3)它将接缝细胞分隔成不同的侧向细胞区室，抑制细胞向背侧和腹侧区室迁移。
engrailed is a homeobox gene essential for developmental functions such as differentiation of cell populations and the onset of compartment boundaries in arthropods and vertebrates. We present the first functional study on engrailed in an unsegmented animal: the nematode Caenorhabditis elegans. In the developing worm embryo, ceh-16/engrailed is predominantly expressed in one bilateral row of epidermal cells (the seam cells). We show that ceh-16/engrailed primes a specification cascade through three mechanisms: (1) it suppresses fusion between seam cells and other epidermal cells by repressing eff-1/fusogen expression; (2) it triggers the differentiation of the seam cells through different factors, including the GATA factor elt-5; and (3) it segregates the seam cells into a distinct lateral cellular compartment, repressing cell migration toward dorsal and ventral compartments.