Training affects muscle phospholipid fatty acid composition in humans
Training affects muscle phospholipid fatty acid composition in humans
复制标题
DOI：
10.1063/1.1380406
复制
发表时间：
2001-02-01
影响因子：
3.3
通讯作者：
Kiens, B
中科院分区：
文献类型：
--
作者：
Helge, JW;Wu, BJ;Kiens, B
训练可以提高胰岛素敏感性，这反过来又可能通过调节燃料供应来影响表现。反过来，胰岛素的作用与骨骼肌中肌肉结构脂质的特定模式有关。本研究探讨了定期运动训练是否对人体肌膜磷脂脂肪酸组成产生影响。7名男性受试者进行单腿膝伸肌耐力训练4周。另一条腿作为对照。术前、术后4天和术后4周，分别对股外侧肌进行肌肉活检。4周后，训练腿中油酸18:1（n-9）和二十二碳六烯酸22:6（n-3）的磷脂脂肪酸含量分别占总脂肪酸的10.9 +/- 0.5%和3.2 +/- 0.4%，显著高于未训练腿（8.8 +/- 0.5%和2.6 +/- 0.4%,P < 0.05）。训练腿n-6和n-3脂肪酸比值（11.1 +/- 0.9）显著低于未训练腿（13.1 +/- 1.2,P < 0.05）。相反，训练不影响肌肉的三酰甘油脂肪酸组成。与未训练腿相比，训练腿的柠檬酸合成酶活性提高了17% （P < 0.05）。在这个模型中，饮食的作用很小，因为饮食摄入量对两条腿的影响是相似的。定期运动训练本身会影响肌膜的磷脂脂肪酸组成，但对肌肉内储存在三酰甘油中的脂肪酸组成没有影响。
Training improves insulin sensitivity, which in turn may affect performance by modulation of fuel availability. Insulin action, in turn, has been linked to specific patterns of muscle structural lipids in skeletal muscle. This study investigated whether regular exercise training exerts an effect on the muscle membrane phospholipid fatty acid composition in humans. Seven male subjects performed endurance training of the knee extensors of one leg for 4 wk. The other leg served as a control. Before, after 4 days, and after 4 wk, muscle biopsies were obtained from the vastus lateralis. After 4 wk, the phospholipid fatty acid contents of oleic acid 18:1(n-9) and docosahexaenoic acid 22:6(n-3) were significantly higher in the trained (10.9 +/- 0.5% and 3.2 +/- 0.4% of total fatty acids, respectively) than the untrained leg (8.8 +/- 0.5% and 2.6 +/- 0.4%, P < 0.05). The ratio between n-6 and n-3 fatty acids was significantly lower in the trained (11.1 +/- 0.9) than the untrained leg (13.1 +/- 1.2, P < 0.05). In contrast, training did not affect muscle triacylglycerol fatty acid composition. Citrate synthase activity was increased by 17% in the trained compared with the untrained leg (P < 0.05). In this model, diet plays a minimal role, as the influence of dietary intake is similar on both legs. Regular exercise training per se influences the phospholipid fatty acid composition of muscle membranes but has no effect on the composition of fatty acids stored in triacylglycerols within the muscle.