A bacterium that degrades and assimilates poly(ethylene terephthalate)
A bacterium that degrades and assimilates poly(ethylene terephthalate)
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DOI：
10.1126/science.aad6359
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发表时间：
2016-03-11
期刊：
影响因子：
56.9
通讯作者：
Oda, Kohei
中科院分区：
文献类型：
--
作者：
Yoshida, Shosuke;Hiraga, Kazumi;Oda, Kohei
聚对苯二甲酸乙二醇酯（PET）在全球塑料制品中广泛使用，其在环境中的累积已成为全球关注的问题。由于人们一直认为能够酶促降解PET的能力仅限于少数几种真菌，生物降解还不是一种可行的修复或回收策略。通过筛选在环境中接触PET的天然微生物群落，我们分离出一种新型细菌，即大阪堺菌（Ideonella sakaiensis）201 - F6，它能够将PET作为其主要的能量和碳源。当在PET上生长时，该菌株产生两种能够水解PET以及反应中间体单（2 - 羟乙基）对苯二甲酸的酶。这两种酶对于将PET有效地酶促转化为其两种对环境无害的单体——对苯二甲酸和乙二醇是必需的。
Poly(ethylene terephthalate) (PET) is used extensively worldwide in plastic products, and its accumulation in the environment has become a global concern. Because the ability to enzymatically degrade PET has been thought to be limited to a few fungal species, biodegradation is not yet a viable remediation or recycling strategy. By screening natural microbial communities exposed to PET in the environment, we isolated a novel bacterium, Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. When grown on PET, this strain produces two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid. Both enzymes are required to enzymatically convert PET efficiently into its two environmentally benign monomers, terephthalic acid and ethylene glycol.