Localization of fibronectin within the renal glomerulus and its production by cultured glomerular cells.
Localization of fibronectin within the renal glomerulus and its production by cultured glomerular cells.
复制标题
纤连蛋白在肾小球内的定位及其通过培养的肾小球细胞的产生。
作者：
Oberley Td;Mosher Df;Mills
用过氧化物酶标记的抗体技术显示肾小球内有纤维连接蛋白。在超微结构水平上，纤维连接蛋白沿着那些邻接毛细血管基底膜的细胞表面，特别是沿着毛细血管上皮细胞足突，存在最大量。细胞内染色也见于肾小球，最明显的是系膜细胞。纤维连接蛋白在肾小球细胞培养物中大量存在于细胞外，并且也在细胞膜和细胞内被证明。
Fibronectin was shown in the kidney glomerulus by the use of the peroxidase-labeled antibody technique. At the ultrastructural level, fibronectin was present in greatest quantities along those cell surfaces that abut the capillary basement membrane, especially along the capillary epithelial cell foot process. Intracellular staining was also seen in the glomerulus, most notably in the mesangial cell. Fibronectin was present extracellularly in large amounts in glomerular cell culture and was also demonstrated on the cell membrane and intracellularly.