Effect of green tea consumption on endothelial function and circulating endothelial progenitor cells in chronic smokers
Effect of green tea consumption on endothelial function and circulating endothelial progenitor cells in chronic smokers
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DOI：
10.1253/circj.70.1052
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Kang, Jung Chaee
中科院分区：
文献类型：
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作者：
Kim, Weon;Jeong, Myung Ho;Kang, Jung Chaee
本研究旨在探讨慢性吸烟者饮用绿色茶对内皮功能和内皮祖细胞的影响及其相互关系。循环EPCs的数量与慢性吸烟和内皮功能障碍呈负相关。绿色茶儿茶素改善内皮功能障碍的慢性smokers.Method和结果在20个年轻的健康吸烟者，内皮功能，定义通过流量介导的内皮依赖性血管舒张（FMD）的肱动脉通过超声以及从外周血中分离的内皮祖细胞的数量，测定在基线和2周后绿色茶消费（8克/天）。循环EPCs通过流式细胞术定量为CD 45 lowCD 34 + KDR 2+细胞，并通过酰基-低密度脂蛋白和异硫氰酸荧光素-凝集素双阳性细胞培养7天后。临床特征和实验室检查结果在基线和绿色茶摄入后2周之间没有显著差异。EPC水平与吸烟数量呈负相关。在饮用绿色茶后2周，通过流式细胞术检测的循环EPCs（78.6 +/- 72.6 vs 156.1 +/- 135.8/ml，p < 0.001）和培养的EPCs（118.2 +/- 35.7 vs 169.31 +/- 58.3/10视野，p < 0.001）迅速增加。2周后FMD显著改善（7.2 +/- 2.8 vs 9.3 +/- 2.4，p < 0.001）。FMD与治疗前和治疗2周后的EPC计数相关（r=0.67，p=0.003）（r=0.60，p=0.013）。结论短期服用绿色茶可迅速改善EPC水平和FMD。饮用绿色茶可能有效预防慢性吸烟者未来的心血管事件。
Background The present study was designed to investigate the effect and relationship of endothelial function and endothelial progenitor cells (EPCs) by green tea consumption in chronic smokers. The numbers of circulating EPCs have an inverse correlation with chronic smoking and endothelial dysfunction. Green tea catechin improved endothelial dysfunction in chronic smokers.Method and Results In 20 young healthy smokers, endothelial functions, defined by flow-mediated endothelium dependent vasodilation (FMD) of the brachial artery via ultrasound as well as the number of EPCs isolated from peripheral blood, were determined at baseline and at 2 weeks after green tea consumption (8 g/day). Circulating EPCs were quantified by flow cytometry as CD45lowCD34+KDR2+ cells and by acyl-low-density lipoprotein and fluorescein isotiocyanate-lectin double positive cells after culture for 7 days. Clinical characteristics and laboratory findings were not significantly different between the baseline and at 2 weeks after green tea intake. EPC levels were inversely correlated with the number of cigarettes smoked. Circulating EPCs by flow cytometry (78.6 +/- 72.6 vs 156.1 +/- 135.8/ml, p < 0.001) and cultured EPCs (118.2 +/- 35.7 vs 169.31 +/- 58.3/10 field, p < 0.001) increased rapidly at 2 weeks after green tea consumption. FMD was significantly improved after 2 weeks (7.2 +/- 2.8 vs 9.3 +/- 2.4, p < 0.001). The FMD correlated with EPC counts (r=0.67, p=0.003) before treatment and after 2 weeks (r=0.60, p=0.013).Conclusions A short-term administration of green tea consumption induces a rapid improvement of EPC levels and FMD. Green tea consumption may be effective to prevent future cardiovascular events in chronic smokers.