Zebrafish genomic library in yeast artificial chromosomes.
Zebrafish genomic library in yeast artificial chromosomes.
复制标题
酵母人工染色体中的斑马鱼基因组文库。
DOI：
10.1006/geno.1997.5155
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Fishman,MC
中科院分区：
文献类型：
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作者：
Zhong,TP;Kaphingst,K;Akella,U;Haldi,M;Lander,ES;Fishman,MC
我们利用从近交系AB斑马鱼中分离的基因组DNA构建了斑马鱼酵母人工染色体（YAC）文库。平均插入片段大小为470 kb，从155个随机选择的YAC的分析估计。该文库由17，000个克隆组成，提供了约4.7倍的斑马鱼基因组覆盖率。将YAC克隆排列在96孔微孔板的各个威尔斯孔中，并合并以允许对整个文库进行快速聚合酶链反应筛选。我们还发现，YAC末端可以很容易地从基于pRML 1/pRML 2的mini-YAC克隆中拯救和测序。
We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones.