Translational intracerebral hemorrhage: a need for transparent descriptions of fresh tissue sampling and preclinical model quality.
Translational intracerebral hemorrhage: a need for transparent descriptions of fresh tissue sampling and preclinical model quality.
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DOI：
10.1007/s12975-015-0399-5
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发表时间：
2015-10
影响因子：
6.9
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Chang CF;Cai L;Wang J
多年来，已经提出了通过提高动物研究质量来提高卒中研究转化成功率的策略。然而，报告临床前脑出血（ICH）研究的文章仍然缺乏对新鲜脑组织采集的足够定性和定量描述。他们也往往缺乏关于动物模型质量的透明度。我们对82篇ICH研究文章进行了系统综述，以确定报告的脑组织采集详细程度。我们发现，只有24例（29%）报告了收集的组织的体积、重量或厚度以及解剖位置的具体描述。因此，高达71%的临床前ICH研究文章没有正确定义如何收集新鲜标本进行生化测量。这种遗漏可能会影响实验室之间结果的重现性。尽管现有标准提高了临床前卒中研究的质量，但ICH研究人员需要确定具体的指南和策略，以避免陷阱，最大限度地减少偏倚，并提高该领域的重现性。
For years, strategies have been proposed to improve translational success in stroke research by improving the quality of animal studies. However, articles that report preclinical intracerebral hemorrhage (ICH) studies continue to lack adequate qualitative and quantitative descriptions of fresh brain tissue collection. They also tend to lack transparency about animal model quality. We conducted a systematic review of 82 ICH research articles to determine the level of detail reported for brain tissue collection. We found that only 24 (29%) reported the volume, weight, or thickness of tissue collected and a specific description of the anatomical location. Thus, up to 71% of preclinical ICH research articles did not properly define how fresh specimens were collected for biochemical measurements. Such omissions may impede reproducibility of results between laboratories. Although existing criteria have improved the quality of preclinical stroke studies, ICH researchers need to identify specific guidelines and strategies to avoid pitfalls, minimize bias, and increase reproducibility in this field.