Vegetable oils rich in alpha linolenic acid increment hepatic n-3 LCPUFA, modulating the fatty acid metabolism and antioxidant response in rats
Vegetable oils rich in alpha linolenic acid increment hepatic n-3 LCPUFA, modulating the fatty acid metabolism and antioxidant response in rats
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DOI：
10.1016/j.plefa.2016.02.002
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发表时间：
2016-08-01
影响因子：
3
通讯作者：
Valenzuela, Alfonso
中科院分区：
文献类型：
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作者：
Angel Rincon-Cervera, Miguel;Valenzuela, Rodrigo;Valenzuela, Alfonso
α-亚麻酸 (C18:3 n-3, ALA) 是一种必需脂肪酸，也是 n-3 家族长链多不饱和脂肪酸 (LCPUFA) 的代谢前体，具有相关的生理和代谢作用：二十碳五烯酸 (C20:5 n-3, EPA) 和二十二碳六烯酸 (C22:6 n-3, DHA）。西方饮食缺乏适当的n-3 LCPUFA摄入量，建议增加此类营养素的膳食供应。富含 ALA 的种子油，例如蔷薇 (Rosa rubiginosa)、印加果 (Plukenetia volubis) 和奇亚籽 (Salvia hispanica) 的种子油，可能是一种值得研究的替代品。本研究评估了喂食葵花籽油 (SFO, 1%) 的大鼠中 n-3 LCPUFA 的肝脏和附睾积聚和生物合成、Delta-5 和 Delta-6 去饱和酶的活性和表达、PPAR-α 和 SREBP-1c 的表达和 DNA 结合活性、氧化应激参数和抗氧化酶的活性 ALA) 作为对照组，菜籽油 (CO, 10% ALA)、蔷薇油 (RMO, 33% ALA)、印加果油 (SIO, 49% ALA) 和奇亚籽油 (ChO, 64% ALA) 作为单一脂质来源。 ALA的大量供应增加了n-3 LCPUFA的增加、去饱和酶的活性和表达、抗氧化状态、PPAR-α的表达和DNA结合、脂肪酸的氧化和抗氧化酶的活性，而SREBP-1c转录因子的表达和DNA结合活性以及脂肪酸的生物合成活性下降。结果表明，富含 ALA 的油（例如 SIO 和 ChO）可能会引发大鼠的代谢反应，例如 n-3 PUFA 产生的代谢反应。 (C) 2016 Elsevier Ltd. 保留所有权利。
Alpha-linolenic acid (C18:3 n-3, ALA) is an essential fatty acid and the metabolic precursor of long-chain polyunsaturated fatty acids (LCPUFA) from the n-3 family with relevant physiological and metabolic roles: eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA). Western diet lacks of suitable intake of n-3 LCPUFA and there are recommendations to increase the dietary supply of such nutrients. Seed oils rich in ALA such as those from rosa mosqueta (Rosa rubiginosa), sacha inchi (Plukenetia volubis) and chia (Salvia hispanica) may constitute an alternative that merits research. This study evaluated hepatic and epididymal accretion and biosynthesis of n-3 LCPUFA, the activity and expression of Delta-5 and Delta-6 desaturase enzymes, the expression and DNA-binding activity of PPAR-alpha and SREBP-1c, oxidative stress parameters and the activity of antioxidative enzymes in rats fed sunflower oil (SFO, 1% ALA) as control group, canola oil (CO, 10% ALA), rosa mosqueta oil (RMO, 33% ALA), sacha inchi oil (SIO, 49% ALA) and chia oil (ChO, 64% ALA) as single lipid source. A larger supply of ALA increased the accretion of n-3 LCPUFA, the activity and expression of desaturases, the antioxidative status, the expression and DNA-binding of PPAR-alpha, the oxidation of fatty acids and the activity of antioxidant enzymes, whereas the expression and DNA-binding activity of SREBP-1c transcription factor and the biosynthetic activity of fatty acids declined. Results showed that oils rich in ALA such as SIO and ChO may trigger metabolic responses in rats such as those produced by n-3 PUFA. (C) 2016 Elsevier Ltd. All rights reserved.