TRANSDUCTION OF LINKED GENETIC CHARACTERS OF THE HOST BY BACTERIOPHAGE-PL
TRANSDUCTION OF LINKED GENETIC CHARACTERS OF THE HOST BY BACTERIOPHAGE-PL
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DOI：
10.1016/0042-6822(55)90016-7
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发表时间：
1955-01-01
期刊：
影响因子：
3.7
通讯作者：
LENNOX, ES
中科院分区：
文献类型：
--
作者：
LENNOX, ES
在Sh上进行了广泛的一系列转导实验。和多种Bact.大肠杆菌B、C、W和K12携带各种遗传标记。所用的引物是P1和突变体P1 kc。成功的转导实现了一些生化标志物，其中选择性的检测方法是可用的。Sh.和几株Bact.杆菌看来供体和受体菌株的育性类型对实现转导的难易程度没有影响。用F-受体和F-或HFr供体，每个噬菌体颗粒的转导频率是相同的。在实验菌株的细菌。coliK 12实例发生在同时转导2个或更多个字符的情况下。这种联合转导的频率似乎是字符之间的连锁的紧密程度的函数。有人试图将P1的溶原化作用与成功的转导联系起来，但结果被从平板上感染细胞背景中释放出来的噬菌体的二次扩散所掩盖。曼恩
An extensive series of transduction experiments were done onSh. dysenteriaeand a variety of strains ofBact. coliB, C, W and K12 carrying an assortment of genetical markers. The phages used were P1 and a nutant P1kc. Successful transductions were achieved with a number of biochemical markers, for which selective methods of detection were available. Transduction occurred betweenSh. dysenteriaeand several of the strains ofBact. coli. It appeared that the fertility types of the donor and acceptor strains had no effect upon the ease or difficulty of achieving transductions. With an F- acceptor and either F- or HFr donors the frequency of transduction per phage particle was the same. In experiments with strains ofBact. coliK12 instances occurred where 2 or more characters were transduced simultaneously. The frequency of such joint transductions appeared to be a function of the closeness of linkage between characters. An attempt was made to correlate lysogenization by P1 with successful transduction, but results were obscured by secondary spread of phage liberated from the background of infected cells on the plates.P. G. Mann.