RGD-coated titanium implants stimulate increased bone formation in vivo
RGD-coated titanium implants stimulate increased bone formation in vivo
复制标题
DOI：
10.1016/s0142-9612(99)00161-1
复制
发表时间：
1999-12-01
期刊：
影响因子：
14
通讯作者：
Valentini, RF
中科院分区：
文献类型：
--
作者：
Ferris, DM;Moodie, GD;Valentini, RF
许多研究表明，肽修饰的表面影响短期和长期的细胞反应，如附着，形状和功能在体外。这些反应通过称为整合素的细胞受体介导，整合素特异性结合来自较大蛋白质的短肽序列。整合素通过几种细胞质信号通路将信息传递到细胞核。然而，关于肽包被表面影响体内细胞反应的能力知之甚少。本研究旨在评价使用金-硫醇化学法在钛棒表面涂覆肽序列Arg-Gly-Asp-Cys（RGDC）并植入大鼠股骨后形成的新骨的质量和数量。垂直于种植体长轴的横截面的组织形态学分析显示，在2周时，在RGD改良种植体周围形成的新骨壳明显比普通种植体厚（26.2 +/- 1.9 μ m vs. 20.5 +/- 2.9 μ m; P < 0.01）。在4周时，也观察到RGD植入物的骨厚度显著增加，而骨周围对照的厚度没有显著变化（32.7 ± 4.6 μ m对22.6 ± 4.0 μ m; P < 0.02）。在4周时进行的机械拔出测试显示，肽改性棒的平均界面剪切强度比对照棒高38%，尽管该差异在统计学上不显著。这些试验数据表明，RGDC肽涂层可以增强钛棒在大鼠股骨中的骨整合。需要在更大的动物模型中对其他肽进行长期研究和评估。(C)1999 Elsevier Science Ltd.保留所有权利。
Numerous studies have demonstrated that peptide modified surfaces influence short- and long-term cell responses such as attachment, shape and function in vitro. These responses are mediated via cell receptors known as integrins which bind specifically to short peptide sequences from larger proteins. Integrins transduce information to the nucleus through several cytoplasmic signalling pathways. Little is known, however, about the ability of peptide-coated surfaces to influence cell responses in vivo. The present study was designed to evaluate the quality and quantity of the new bone formed in response to titanium rods surface-coated with the peptide sequence Arg-Gly-Asp-Cys (RGDC) using gold-thiol chemistry and implanted in rat femurs. Histomorphometric analysis of cross-sections perpendicular to the implant long axis showed a significantly thicker shell of new bone formed around RGD-modified Versus plain implants at 2 weeks (26.2 +/- 1.9 vs. 20.5 +/- 2.9 mu m; P < 0.01). A significant increase in bone thickness for RGD implants was also observed at 4 weeks while bone surrounding controls did not change significantly in thickness (32.7 +/- 4.6 vs. 22.6 +/- 4.0 mu m; P < 0.02). Mechanical pull-out testing conducted at 4 weeks revealed the average interfacial shear strength of peptide modified rods was 38% greater than control rods although this difference was not statistically significant. These pilot data suggest that an RGDC peptide coating may enhance titanium rod osseointegration in the rat femur. Long-term studies and evaluation of other peptides in larger animal models are warranted. (C) 1999 Elsevier Science Ltd. All rights reserved.