ISOLATION OF HUMAN CDNA CLONES OF MYB-RELATED GENES, A-MYB AND B-MYB
ISOLATION OF HUMAN CDNA CLONES OF MYB-RELATED GENES, A-MYB AND B-MYB
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DOI：
10.1093/nar/16.23.11075
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发表时间：
1988-12-09
影响因子：
14.9
通讯作者：
ISHIZAKI, R
中科院分区：
文献类型：
--
作者：
NOMURA, N;TAKAHASHI, M;ISHIZAKI, R
通过筛选人cDNA文库获得了myb相关基因A-myb和B-myb的cDNA克隆。B-myb的开放阅读框架可编码700个氨基酸残基的蛋白质。虽然C-末端还没有被克隆，但已确定了A-myb的几乎整个编码区，其长度为745个氨基酸。A-myb和B-myb蛋白在三个区域与myb蛋白高度同源。结构域I长161个氨基酸，在myb基因家族中是非常保守的。人myb与A-myb在结构域I的氨基酸同源性为90%。约85个氨基酸长的结构域II保守性较差。虽然它是一个很短的延伸，结构域III被发现在C-末端区域。A-myb和B-myb的mRNA分别为5.0和2.6kb。在各种肿瘤的myb基因家族的mRNA表达模式。
cDNA clones of the myb-related genes A-myb and B-myb were obtained by screening human cDNA libraries. The predicted open reading frame of B-myb could encode a protein of 700 amino acid residues. Although the C-terminal end has not been cloned yet, an almost entire coding region of A-myb which is 745 amino acid long, was determined. The A-myb and B-myb proteins are highly homologous with the myb protein in three regions. Domain I, which is 161 amino acid long, is well conserved in the myb gene family. The homology between human-myb and A-myb in domain I is 90% at the amino acid level. Domain II, which is about 85 amino acid long, is less well conserved. Although it is a short stretch, domain III is found in the C-terminal region. The mRNAs of A-myb and B-myb were 5.0 and 2.6 kb, respectively. The mRNA expression pattern of the myb gene family in various tumors is presented.