Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice.
Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice.
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DOI：
10.1038/s41514-022-00089-8
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发表时间：
2022-06-27
期刊：
影响因子：
--
通讯作者：
de Cabo, Rafael
中科院分区：
文献类型：
--
作者：
Bernier, Michel;Enamorado, Ignacio Navas;Gomez-Cabrera, Mari Carmen;Calvo-Rubio, Miguel;Gonzalez-Reyes, Jose Antonio;Price, Nathan L;Cortes-Rodriguez, Ana Belen;Rodriguez-Aguilera, Juan Carlos;Rodriguez-Lopez, Sandra;Mitchell, Sarah J;Murt, Kelsey N;Kalafut, Krystle;Williams, Katrina M;Ward, Christopher W;Stains, Joseph P;Brea-Calvo, Gloria;Villalba, Jose M;Cortassa, Sonia;Aon, Miguel A;de Cabo, Rafael
随着年龄的增长，骨骼肌适应不同的运动训练方式;然而，这两个变量在系统和组织水平上的影响还没有完全了解。在这里，成年和老年C57 BL/6雄性小鼠被分配到三组之一：久坐，每天高强度间歇训练（HIIT），或中等强度连续训练（MICT）4周，与老年组的运动能力相适应。在MICT老年组中主要观察到身体组成、空腹血糖和肌肉力量的改善，而HIIT训练对成年和老年动物的影响不太清楚。通过电子显微镜、OXPHOS测定、呼吸测定和肌肉蛋白生物标志物显示，骨骼肌对运动训练表现出结构和功能适应性。腓肠肌转录组学结合肝脏和血清代谢组学分析揭示了运动训练对年龄依赖性代谢重构的响应。这些结果支持了一种量身定制的运动处方方法，旨在改善老年人的健康状况，改善与年龄相关的肌肉力量和功能丧失。
Skeletal muscle adapts to different exercise training modalities with age; however, the impact of both variables at the systemic and tissue levels is not fully understood. Here, adult and old C57BL/6 male mice were assigned to one of three groups: sedentary, daily high-intensity intermittent training (HIIT), or moderate intensity continuous training (MICT) for 4 weeks, compatible with the older group’s exercise capacity. Improvements in body composition, fasting blood glucose, and muscle strength were mostly observed in the MICT old group, while effects of HIIT training in adult and old animals was less clear. Skeletal muscle exhibited structural and functional adaptations to exercise training, as revealed by electron microscopy, OXPHOS assays, respirometry, and muscle protein biomarkers. Transcriptomics analysis of gastrocnemius muscle combined with liver and serum metabolomics unveiled an age-dependent metabolic remodeling in response to exercise training. These results support a tailored exercise prescription approach aimed at improving health and ameliorating age-associated loss of muscle strength and function in the elderly.
影响因子：
13.1
作者：
Navas-Enamorado I;Bernier M;Brea-Calvo G;de Cabo R
通讯作者：
de Cabo R