The distinction between gamma-glutamylhydroxamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues.
The distinction between gamma-glutamylhydroxamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues.
复制标题
大鼠组织中γ-谷氨酰异羟肟酸合成酶和L-谷氨酰胺-羟胺谷氨酰转移酶活性的区别。
DOI：
10.1042/bj1330059
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
N. A. Estes
中科院分区：
文献类型：
--
作者：
A. Herzfeld;N. A. Estes
γ-谷氨酰异羟肟酸形成的匀浆在最佳的测定条件下，利用L-谷氨酸和ATP（γ-谷氨酰异羟肟酸合成酶）和L-谷氨酰胺和ADP（L-谷氨酰胺-羟胺谷氨酰转移酶）在一些正常和肿瘤大鼠组织中的酶活性的比例不稳定。虽然γ-谷氨酰异羟肟酸合成酶活性在成年肝脏和肾脏是相同的男性和女性，L-谷氨酰胺-羟胺谷氨酰转移酶活性在女性的器官显着降低。这两种活性在肝、肾、脑和肌肉中的发育形成不是同时的。甲状腺素可提前诱发胎肝或新生儿肾中L-谷氨酰胺-羟胺谷氨酰转移酶活性，但γ-谷氨酰异羟肟酸合成酶活性保持不变。注射皮质醇对胸腺和肝癌的两种活性也有不同的影响。不一致的组织分布，异步发育的形成和不同的反应，几种激素刺激提供的证据表明，在体内的两个活动不是由相同的蛋白质催化。
The formation of gamma-glutamylhydroxamate by homogenates under optimum assay condition showed an inconstancy in the ratios of the enzyme activities utilizing l-glutamate and ATP (gamma-glutamylhydroxamate synthetase) and l-glutamine and ADP (l-glutamine-hydroxylamine glutamyltransferase) in a number of normal and neoplastic rat tissues. Although gamma-glutamylhydroxamate synthetase activities in adult livers and kidneys were identical in males and females, l-glutamine-hydroxylamine glutamyltransferase activities in the organs of females were significantly lower. The developmental formations of the two activities in liver, kidney, brain and muscle were not simultaneous. The l-glutamine-hydroxylamine glutamyltransferase activity in foetal liver or neonatal kidney could be prematurely evoked by thyroxine, but the gamma-glutamylhydroxamate synthetase activity remained unchanged. Injections of cortisol also had dissimilar effects on the two activities in thymus and hepatomas. The discrepant tissue distribution, asynchronous developmental formation and differential response to several hormonal stimuli provide evidence in vivo that the two activities are not catalysed by the same protein.