Characterisation of cDNA and genomic clones encoding homologues of the 65 kDa regulatory subunit of protein phosphatase 2A in Arabidopsis thaliana
Characterisation of cDNA and genomic clones encoding homologues of the 65 kDa regulatory subunit of protein phosphatase 2A in Arabidopsis thaliana
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拟南芥中编码蛋白磷酸酶 2A 65 kDa 调节亚基同源物的 cDNA 和基因组克隆的表征
DOI：
10.1007/bf00040694
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发表时间：
1994
影响因子：
5.1
通讯作者：
I. M. Evans
中科院分区：
文献类型：
--
作者：
A. Slabas;A. Fordham;Daniel Fletcher;J. M. Martínez;R. Swinhoe;R. Croy;I. M. Evans
从拟南芥（Arabidopsis thaliana）cDNA文库中分离到两个与蛋白磷酸酶2A（PP 2A）65 kDa调节亚基（PR 65）同源的cDNA编码序列。它们被命名为pDF 1和pDF 2。pDF 1长1795 bp，与人和猪PP 2A调节亚基序列相比，代表全长克隆。它编码587个氨基酸残基的预测多肽。pDF 2在5′端截短了237 bp。已确定两种cDNA物种的完整核苷酸序列。核苷酸和推导的氨基酸序列的比较表明，这两个序列是同源的，但不相同，因此必须来自两个不同的基因。对甘蓝型油菜（Brassica napus L.）不同发育时期种子和幼叶的总RNA和poly（A）+ RNA进行了北方杂交分析。（油菜）。这两个cDNA探针杂交到一个单一的主要mRNA物种的CA。2.2 KB.油菜花后33 d左右，种子总RNA表达量最高，种子poly（A）+ RNA的转录水平高于油菜幼叶。用Southern杂交技术对两个A. thaliana和B. napus基因组DNA;这确定了A.在B中，一个由至少2或3个成员组成的大的多基因家族。从A. napus中分离到两个独立的基因组克隆，每个基因组克隆的成员数至少为5或6个。拟南芥基因组文库对两者共有的片段进行测序，结果表明两个克隆的序列相同，因此，假定它们含有相同的基因组序列。所选择的基因组序列，命名为regA，长3639 bp，编码序列含有11个内含子。该基因编码590个氨基酸残基的预测多肽。与两个cDNA序列的序列比较表明，它与两者同源，但不完全相同，证实了至少有三个不同的基因存在于A。thaliana中表达。
Two cDNA species encoding sequences homologous to the 65 kDa regulatory subunit (PR 65) of protein phosphatase 2A (PP2A) have been isolated from an Arabidopsis thaliana cDNA library. These were designated pDF1 and pDF2. pDF1 is 1795 bp long and by comparison with the human and porcine PP2A regulatory subunit sequences represents a full-length clone. It encodes a predicted polypeptide of 587 amino acid residues. pDF2 is truncated at the 5′ end by 237 bp. The complete nucleotide sequences have been determined for both cDNA species. Comparison of the nucleotide and the deduced amino acid sequences showed that the two sequences were homologous but not identical and therefore must be derived from two different genes. Northern blot analysis was performed on total RNA and poly(A)+ RNA isolated from seed at various stages of development and from young leaf material of Brassica napus L. (oilseed rape). Both cDNA probes hybridised to a single major mRNA species of ca. 2.2 kb. The highest level of expression was observed in the total RNA from developing rape seed at about 33 days after flowering, and the transcript level in the poly(A)+ RNA of the seed was higher than in young leaf of oilseed rape. Southern blot analysis was performed on two varieties of A. thaliana and B. napus genomic DNA; this identified a small family of genes in A. thaliana consisting of at least 2 or 3 members and a larger multigene family in B. napus of at least 5 or 6 members.Two independent genomic clones were isolated from an A. thaliana genomic library. Sequencing of a fragment common to both revealed that the sequence was identical in both clones and, therefore, they were assumed to contain the same genomic sequence. The genomic sequence selected, designated regA, is 3639 bp long and the coding sequence contains eleven introns. The gene encodes a predicted polypeptide of 590 amino acid residues. The sequence comparison with both cDNA sequences showed that it is homologous but not identical to the two, confirming that at least three different genes exist in A. thaliana which encode PR65 of PP2A.
DOI：
--
发表时间：
1989-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Cohen;P. Cohen
通讯作者：
P. Cohen;P. Cohen
影响因子：
11.4
作者：
LUTCKE, HA;CHOW, KC;SCHEELE, GA
通讯作者：
SCHEELE, GA
蛋白磷酸酶 2A A 亚基的表达及其与催化和调节亚基相互作用的表征。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kamibayashi,C;Lickteig,RL;Estes,R;Walter,G;Mumby,MC
通讯作者：
Mumby,MC