ELECTRON MICROSCOPE AND X-RAY DIFFRACTION STUDIES OF THE EFFECTS OF DEHYDRATION ON THE STRUCTURE OF NERVE MYELIN .1. PERIPHERAL NERVE
ELECTRON MICROSCOPE AND X-RAY DIFFRACTION STUDIES OF THE EFFECTS OF DEHYDRATION ON THE STRUCTURE OF NERVE MYELIN .1. PERIPHERAL NERVE
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DOI：
10.1083/jcb.8.1.13
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发表时间：
1960-01-01
期刊：
影响因子：
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通讯作者：
FINEAN, JB
中科院分区：
文献类型：
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作者：
FINEAN, JB
青蛙坐骨神经的脱水已被研究，使标本成为部分或完全干燥，然后固定和准备电子显微镜。CsO4固定的制剂的低倍电子显微照片显示出显着的组织收缩，这可以与初步干燥过程中的水分损失定量。KMnC4固定似乎导致干燥组织的再水化。CsO 4固定制剂的更高倍率电子显微镜照片显示了髓鞘层的一系列修饰，这可能与干燥过程中记录的小角X射线衍射数据的变化相关。干燥的中间阶段的特点是层的部分塌陷和在髓鞘的某些区域的intraperiod致密线的消失。在整个鞘管中保持塌陷层和非塌陷层之间的连续性。完全干燥的制剂显示出髓磷脂层的两个主要变化。在许多地区的层（主层）类似于那些正常的准备工作，但表现出强化和经常加倍的intracperiod密集线。此外，有一个非常广泛的系统的罚款（40埃周期）致密层，其中一些可以证明是连续的主要层。在这种情况下，观察到两个精细层与每个主层相关。衍射数据和电子显微镜数据之间的相关性进行了讨论，并作出了一些推测有关的分子意义的意见。
The dehydration of frog sciatic nerve has been studied by allowing specimens to become partially or fully dried before fixation and preparation for electron microscopy. Low magnification electron micrographs of CsO4-fixed preparations showed marked tissue shrinkage which could be correlated quantitatively with the loss of water during the preliminary drying. KMnC4-fixation appeared to cause a rehydration of the dried tissue. Higher magnification electron micrographs of the CsO4-fixed preparations showed a sequence of modifications of the myelin layers which could be correlated with changes in the small-angle X-ray diffraction data which were recorded during drying. An intermediate stage of drying was characterised by a partial collapse of layers and a disappearance of the intraperiod dense line in some regions of the myelin sheath. Continuity between collapsed and non-collapsed layers was maintained throughout the sheath. The fully dried preparation showed two main modifications of the myelin layers. In many regions the layers (principal layers) resembled those of normal preparations, but showed an intensification and frequently a doubling of the intraperiod dense line. In addition, there was a very extensive system of fine (40 A periodicity) dense layers, some of which could be demonstrated to be continuous with the principal layers. In such cases it was observed that two of the fine layers were related to each principal layers. The correlation between diffraction data and electron microscope data is discussed, and some speculations are made concerning the molecular significance of the observations.