RELEASE OF SIMIAN VIRUS-40 VIRIONS FROM EPITHELIAL-CELLS IS POLARIZED AND OCCURS WITHOUT CELL-LYSIS
RELEASE OF SIMIAN VIRUS-40 VIRIONS FROM EPITHELIAL-CELLS IS POLARIZED AND OCCURS WITHOUT CELL-LYSIS
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DOI：
10.1128/jvi.63.5.2278-2288.1989
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发表时间：
1989-05-01
影响因子：
5.4
通讯作者：
COMPANS, RW
中科院分区：
文献类型：
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作者：
CLAYSON, ET;BRANDO, LVJ;COMPANS, RW
我们研究了猴病毒40（SV 40）病毒粒子从几种猴肾细胞系中释放的过程。在检查的所有细胞系中，在出现任何显著致细胞病变效应之前，均观察到高水平的病毒释放，表明SV 40利用了一种不涉及细胞裂解的宿主细胞逃逸机制。我们证明，SV 40释放极化的两种上皮细胞类型（Vero C1008和主要非洲绿色猴肾细胞）生长在可渗透的支持;释放的病毒几乎只发生在顶端表面。相反，从非极化CV-1细胞的顶端和基底培养液中回收了等效的SV 40病毒体。SV 40病毒粒子在上皮细胞的顶端表面和细胞质的平滑膜囊泡中观察到大量。钠离子载体莫能菌素，囊泡运输的抑制剂，被发现抑制SV 40的释放，而不改变病毒蛋白质的合成或感染病毒的生产。
We have investigated the process of release of simian virus 40 (SV40) virions from several monkey kidney cell lines. High levels of virus release were observed prior to any significantly cytopathic effects in all cell lines examined, indicating that SV40 utilizes a mechanism for escape from the host cell which does not involve cell lysis. We demonstrate that SV40 release was polarized in two epithelial cell types (Vero C1008 and primary African green monkey kidney cells) grown on permeable supports; release of virus occurs almost exclusively at apical surfaces. In contrast, equivalent of SV40 virions were recovered from apical and basal culture fluids of nonpolarized CV-1 cells. SV40 virions were observed in large numbers on apical surfaces of epithelial cells and in cytoplasmic smooth membrane vesicles. The sodium ionophore monensin, an inhibitor of vesicular transport, was found to inhibit SV40 release without altering viral protein synthesis or infections virus production.