Atypical laminin spots and pull- generated microtubule-actin projections mediate Drosophila wing adhesion
Atypical laminin spots and pull- generated microtubule-actin projections mediate Drosophila wing adhesion
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DOI：
10.1016/j.celrep.2021.109667
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Jose C. Pastor-Pareja
中科院分区：
文献类型：
--
作者：
Tianhui Sun;Yuzhao Song;Dequn Teng;Yanan Chen;Jianli Dai;Mengqi Ma;Wei Zhang;Jose C. Pastor-Pareja
During Drosophila metamorphosis, dorsal and ventral wing surfaces adhere, separate, and reappose in a paradoxical process involving cell-matrix adhesion, matrix production and degradation, and long cellular projections. The identity of the intervening matrix, the logic behind the adhesion-reapposition cycle, and the role of projections are unknown. We find that laminin matrix spots devoid of other main basement membrane components mediate wing adhesion. Through live imaging, we show that long microtubule-actin cables grow from those adhesion spots because of hydrostatic pressure that pushes wing surfaces part. Formation of cables resistant to pressure requires spectraplakin, Patronin, septins, and Sdb, a SAXO1/2 microtubule stabilizer expressed under control of wing intervein-selector SRF. Silkworms and dead-leaf butterflies display similar dorso-ventral projections and expression of Sdb in intervein SRF-like patterns. Our study supports the morphogenetic importance of atypical basement-membrane-related matrices and dissects matrix-cytoskeleton coordination in a process of great evolutionary significance.
