Prp43: An RNA helicase-like factor involved in spliceosome disassembly
Prp43: An RNA helicase-like factor involved in spliceosome disassembly
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DOI：
10.1073/pnas.94.22.11798
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发表时间：
1997-10-28
影响因子：
11.1
通讯作者：
Abelson, JN
中科院分区：
文献类型：
--
作者：
Arenas, JE;Abelson, JN
酿酒酵母基因PRP 2、PRP 16和PRP 22编码属于高度保守的推定RNA解旋酶“DEAH "家族的前mRNA剪接因子。我们先前鉴定了该家族的另外两个成员，JA 1和JA 2。为了研究其生物学功能，我们克隆了JA 1基因，并产生了携带与DEAR家族其他成员高度保守区域中发现的突变相同的突变的等位基因。一个ja 1等位基因携带的突变相同的温度敏感（ts）prp 22 -1基因赋予ts表型时，整合到一个温和型菌株的基因组中的基因置换，北方分析的RNA从ts菌株转移到一个非允许的温度显示积累的未剪接的前mRNA和切除的内含子lariats。此外，剪接复合物的分析表明，内含子lariats积累在剪接体中，这些结果表明，MI编码的前mRNA加工因子（Prp）参与剪接体的解体后，成熟的mRNA释放。因此，我们将该基因重命名为PRP 43。
The Saccharomyces cerevisiae genes PRP2, PRP16, and PRP22 encode pre-mRNA splicing factors that belong to the highly conserved ''DEAH'' family of putative RNA helicases, We previously identified two additional members of this Family, JA1 and JA2. To investigate its biological function, we cloned the JA1 gene and generated alleles carrying mutations identical to those found in highly conserved regions of other members of the DEAR family. A ja1 allele carrying a mutation identical to that in the temperature-sensitive (ts) prp22-1 gene conferred ts phenotype when integrated into the genome of a mild-type strain by gene replacement, Northern analysis of RNA obtained from the ts strain shifted to a nonpermissive temperature revealed accumulation of unspliced pre-mRNAs and excised intron lariats. Furthermore, analysis of splicing complexes showed that intron lariats accumulated in spliceosomes, The results presented indicate that MI encodes a pre-mRNA processing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA. We have therefore renamed this gene PRP43.