Effects of follicle‐stimulating hormone followed by gonadotropin‐releasing hormone on embryo production by ovum pick‐up and in vitro fertilization in the river buffalo ( Bubalus bubalis )
Effects of follicle‐stimulating hormone followed by gonadotropin‐releasing hormone on embryo production by ovum pick‐up and in vitro fertilization in the river buffalo ( Bubalus bubalis )
复制标题
促卵泡激素和促性腺激素释放激素对水牛（Bubalus bubalis）取卵和体外受精胚胎产生的影响
DOI：
10.1111/asj.13196
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发表时间：
2019
影响因子：
2
通讯作者：
Nagano Masashi
中科院分区：
文献类型：
--
作者：
Sakaguchi Kenichiro;Maylem Excel Rio S.;Tilwani Ramesh C.;Yanagawa Yojiro;Katagiri Seiji;Atabay Edwin C.;Atabay Eufrocina P.;Nagano Masashi
在本研究中，我们通过超声引导取卵（OPU）和体外受精（IVF）检查了使用促卵泡激素（FSH）然后使用促性腺激素释放激素（GnRH）的超刺激对布法罗胚胎生产的影响。9头Murrah水牛接受了无超刺激的OPU-IVF（对照）。对收集的卵母细胞的形态进行评价，然后将卵母细胞进行体外成熟（IVM）。在OPU后两天，对相同的9头水牛每天注射两次FSH，持续3天进行超刺激，然后注射GnRH。在注射GnRH后23-24小时通过OPU收集卵母细胞并提交至IVM（超刺激组）。超刺激组的卵泡总数、直径> 8 mm的卵泡数和被多层卵丘细胞包围的卵母细胞数均高于对照组（p≤ 0.05）。IVF后超刺激组卵裂率和囊胚发育率均高于对照组（p< 0.05）。总之，超刺激提高了水牛卵母细胞的质量和OPU-IVF的胚胎生产力。
In this study, we examined the effects of superstimulation using follicle‐stimulating hormone (FSH) followed by gonadotropin‐releasing hormone (GnRH) on buffalo embryo production by ultrasound‐guided ovum pick‐up (OPU) and in vitro fertilization (IVF). Nine Murrah buffaloes were subjected to OPU‐IVF without superstimulation (control). The morphologies of the oocytes collected were evaluated, and oocytes were then submitted to in vitro maturation (IVM). Two days after OPU, same nine buffaloes were treated with twice‐daily injections of FSH for 3 days for superstimulation followed by a GnRH injection. Oocytes were collected by OPU 23–24 hr after the GnRH injection and submitted to IVM (the superstimulated group). The total number of follicles, number of follicles with a diameter > 8 mm, and number of oocytes surrounded by multi‐layered cumulus cells were higher in the superstimulated group than in the control group (p≤ 0.05). After IVF, the percentages of cleavage and development to blastocysts were higher in the superstimulated group than in the control group (p< 0.05). In conclusion, superstimulation improved the quality of oocytes and the embryo productivity of OPU‐IVF in river buffaloes.