A HUMAN C-ERBA ONCOGENE HOMOLOG IS CLOSELY PROXIMAL TO THE CHROMOSOME 17 BREAKPOINT IN ACUTE PROMYELOCYTIC LEUKEMIA
A HUMAN C-ERBA ONCOGENE HOMOLOG IS CLOSELY PROXIMAL TO THE CHROMOSOME 17 BREAKPOINT IN ACUTE PROMYELOCYTIC LEUKEMIA
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DOI：
10.1073/pnas.81.14.4495
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
CROCE, CM
中科院分区：
文献类型：
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作者：
DAYTON, AI;SELDEN, JR;CROCE, CM
A human c[complementary]DNA library was screened for sequences homologous to the erbA gene of avian erythroblastosis virus (AEV). One such clone, cHerbA-1, was used to map the chromosomal location of highly homologous human sequences that were found to be present on chromosome 17 as judged by Southern blot screening of a panel of mouse-human hybrid cell lines segregating human chromosomes. cHerbA-1 was hybridized in situ to metaphase chromosomes from a normal male subject and from a female patient with an acute promyelocytic leukemia (APL) having the typical t(15;17) translocation. The results localized the cellular c-erbA sequences on chromosome 17 to the q21-q24 region of normal chromosomes and indicated that the c-erbA sequences remained on the 17q- chromosome in the APL cells, suggesting that they could be assigned to the 17(q21-q22) region. For additional data, human neoplastic cells derived from a poorly differentiated acute leukemia carrying a t(17;21) translocation were hybridized with thymidine kinase(TK)-deficient LMTK- mouse cells. A resulting hybrid, containing only the 21q+ chromosome, did not have human c-erbA sequences. Since the breakpoint on 17q in this translocation was similar to that in the APL t(15;17) translocation, this supported the assignment of c-erbA to the q21-q22 region of chromosome 17. The apparent close proximity of the c-erbA sequences to the chromosomal breakpoints in these 2 leukemias suggests a possible role for this oncogene homologue in the development of these neoplasms.
