CLINICAL APPLICATION OF BLOOD TRANSKETOLASE DETERMINATIONS
CLINICAL APPLICATION OF BLOOD TRANSKETOLASE DETERMINATIONS
复制标题
DOI：
10.1056/nejm196209202671204
复制
发表时间：
1962-01-01
影响因子：
158.5
通讯作者：
DREYFUS, PM
中科院分区：
文献类型：
--
作者：
DREYFUS, PM
初步观察表明，全血转酮醇酶不仅反映了严重的，但也最低程度的硫胺素缺乏症。酶的活性似乎直接随可用辅因子的水平而变化。以每小时每毫升全血中景天庚酮糖-7-磷酸的微克数表示的酶活性在不同受试者之间或在一天中的不同时间之间可能有很大差异，这取决于测试与食物摄入的关系。该酶的活性会受到可能不是硫胺素缺乏症的代谢条件的抑制，例如酸中毒或肝脏或肾脏疾病。事实上，大浓度的TPP在体外添加的是无法恢复转酮酶活性的正常水平在几天内开始耗尽表明，在进行性硫胺素缺乏症可能是一个逐渐耗尽的辅酶和脱辅基酶。
Preliminary observations show that whole-blood transketolase reflects not only severe but also minimal degree of thiamine deficiency. The activity of the enzyme appears to vary directly with the levels of available co-factor. The enzymatic activity as expressed in micrograms of sedo-heptulose- 7-phosphate per milliliter of whole blood per hour may well differ from subject to subject or from one time of the day to another, depending upon the relation of the test to the intake of food. The activity of the enzyme is depressed by metabolic conditions possibly other than thiamine deficiency, such as acidosis or liver or renal disease. The fact that large concentrations of TPP added in vitro are unable to restore transketolase activity to normal levels within a few days of the onset of depletion suggests that in progressive thiamine deficiency there may be a gradual depletion of both coenzyme and apoenzyme.