In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano-Hydroxyapatite/Collagen Scaffold
In Vitro Biocompatibility and Osteoblast Differentiation of an Injectable Chitosan/Nano-Hydroxyapatite/Collagen Scaffold
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可注射壳聚糖/纳米羟基磷灰石/胶原支架的体外生物相容性和成骨细胞分化
DOI：
10.1155/2012/401084
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Yu, Bo
中科院分区：
文献类型：
--
作者：
Chen, Yan;Huang, Zhi;Yu, Bo
本研究的目的是评估由壳聚糖（CS）、纳米羟基磷灰石和胶原（nHAC）组成的原位形成复合材料的体外细胞生物相容性，该复合材料具有类似于天然骨的复杂分级结构。将MC 3 T3- E1小鼠颅骨前成骨细胞培养在可注射CS/nHAC和CS支架表面。研究接种的MC 3 T3-E1的增殖10天。细胞毒性，细胞增殖，成骨标志物，如碱性磷酸酶（ALP），1型胶原（COL-1），RUNX-2，和骨钙素（OCN）的细胞表达通过生化测定和逆转录聚合酶链反应进行了检查。两个支架组之间的细胞活力和总细胞构成（通过dsDNA测量）相似。然而，当成骨细胞在CS/nHAC支架上培养时，ALP、COL-1、OCN和RUNX-2的产生显著更大。CS/nHAC支架上成骨标志物产生的增加表明这些支架在促进成骨细胞矿化方面上级仅壳聚糖支架。这些结果表明CS/nHAC支架在骨组织工程中的应用潜力。
The purpose of this study was to evaluate the in vitro cell biocompatibility of an in situ forming composite consisting of chitosan (CS), nano-hydroxyapatite and collagen (nHAC), which has a complex hierarchical structure similar to natural bone. MC3T3- E1 mouse calvarial preosteoblasts were cultured on the surface of the injectable CS/nHAC and CS scaffold. The proliferations of seeded MC3T3-E1 were investigated for 10 days. Cytotoxicity, cell proliferation, and cell expression of osteogenic markers such as alkaline phosphatase (ALP), type 1 collagen (COL-1), RUNX-2, and osteocalcin (OCN) were examined by biochemical assay and reverse transcription polymerase chain reaction. Cell viability and total cellularity (measured by dsDNA) were similar between the two scaffold groups. However, ALP, COL-1, OCN, and RUNX-2 production were significantly greater when osteoblasts were cultured on CS/nHAC scaffolds. The increase in osteogenic markers production on CS/nHAC scaffolds indicated that these scaffolds were superior to chitosan-only scaffolds in facilitating osteoblast mineralization. These results demonstrate the potential of the CS/nHAC scaffolds to be used in bone tissue engineering.